The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jul. 10, 2018

Filed:

Jan. 03, 2017
Applicants:

Mitsui Chemicals, Inc., Tokyo, JP;

Meiji Seika Pharma Co., Ltd., Tokyo, JP;

Inventors:

Hisaaki Yanai, Mobara, JP;

Hiroki Tamai, Ichihara, JP;

Masami Osabe, Chiba, JP;

Fumikazu Yokoyama, Yokohama, JP;

Kaoru Okakura, Odawara, JP;

Atsushi Inoue, Odawara, JP;

Assignees:
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 15/00 (2006.01); C12N 9/24 (2006.01); C12N 9/42 (2006.01); C12N 15/64 (2006.01); C12P 19/04 (2006.01); C07H 21/04 (2006.01); C12P 19/14 (2006.01); C11D 3/386 (2006.01); A21D 8/04 (2006.01); C12P 19/02 (2006.01); C13K 1/02 (2006.01); C13K 13/00 (2006.01); A23K 20/189 (2016.01);
U.S. Cl.
CPC ...
C12N 9/2482 (2013.01); A21D 8/042 (2013.01); A23K 20/189 (2016.05); C11D 3/38636 (2013.01); C12N 9/248 (2013.01); C12P 19/02 (2013.01); C12P 19/14 (2013.01); C12Y 302/01004 (2013.01); C12Y 302/01008 (2013.01); C13K 1/02 (2013.01); C13K 13/002 (2013.01); C12Y 302/01021 (2013.01); Y02E 50/16 (2013.01); Y02P 20/52 (2015.11);
Abstract

What is aimed at is provision of an inexpensive and efficient saccharification method for lignocellulose using a thermostable xylanase and provision of a mutant xylanase that has a substitute amino acid residue, and that exhibits stable activity even under severe conditions in which enzymes easily inactivate, and that provides an initial rate of reaction not significantly reduced as compared to a wild-type xylanase corresponding to the mutant xylanase. Provided is a method of producing a saccharified product of lignocellulose, including contacting a lignocellulosic raw material with a thermostable xylanase, and a mutant xylanase that provides an initial rate of reaction that is at least 70% of that provided by a wild-type xylanase corresponding thereto, that has a xylanase activity after heat treatment at 50° C. for 24 hours that is at least 50% of its xylanase activity before the heat treatment, and that has a substitute amino acid residue.


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