The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 10, 2018
Filed:
Jul. 18, 2013
Applicant:
Singapore Health Services Pte Ltd, Singapore, SG;
Inventors:
Assignee:
SINGAPORE HEALTH SERVICES PTE LTD, Singapore, SG;
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/079 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0621 (2013.01); C12N 2500/24 (2013.01); C12N 2500/25 (2013.01); C12N 2500/30 (2013.01); C12N 2500/32 (2013.01); C12N 2500/34 (2013.01); C12N 2500/38 (2013.01); C12N 2500/62 (2013.01); C12N 2500/72 (2013.01); C12N 2500/80 (2013.01); C12N 2501/06 (2013.01); C12N 2501/10 (2013.01); C12N 2501/31 (2013.01); C12N 2501/33 (2013.01); C12N 2501/39 (2013.01);
Abstract
The invention relates to a dual-media approach for culturing isolated corneal endothelial cells. Isolated corneal endothelial cells are first contacted with a proliferative medium to propagate and/or expand the endothelial cells followed by a maintenance medium to preserve the morphology and/or characteristics of the corneal endothelial cells. The invention includes the proliferative medium and the maintenance medium and also a combination of the two medium.