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The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Patent No.:

US 10011850 B1

PDFFull Text
Date of Patent:
Jul. 03, 2018

Filed:

Apr. 23, 2014

Using rna-guided foki nucleases (rfns) to increase specificity for rna-guided genome editing

Applicant:

The General Hospital Corporation, Boston, MA (US);

Inventors:

J. Keith Joung, Winchester, MA (US);

Shengdar Tsai, Charlestown, MA (US);

Assignee:

The General Hospital Corporation, Boston, MA (US);

Attorney:

Fish & Richardson P.C.

Primary Examiner:

Suzanne M Noakes

Assistant Examiner:

Jae W Lee

Int. Cl.
CPC ...
C12N 15/85 (2006.01); C12N 9/22 (2006.01); C12N 15/63 (2006.01); C07K 14/005 (2006.01); C07K 14/195 (2006.01); C12N 9/02 (2006.01); C12N 9/10 (2006.01); C12N 9/16 (2006.01); C12N 9/96 (2006.01); C12N 15/04 (2006.01); C12N 15/90 (2006.01); C12N 15/10 (2006.01);
U.S. Cl.
CPC ...
C12N 15/907 (2013.01); C12N 9/22 (2013.01); C12N 15/102 (2013.01); C07K 2319/00 (2013.01); C12N 2310/20 (2017.05); C12N 2800/80 (2013.01); C12Y 301/00 (2013.01); C12Y 301/21004 (2013.01);
Abstract

Many studies have shown that CRISPR-Cas nucleases can tolerate up to five mismatches and still cleave; it is hard to predict the effects of any given single or combination of mismatches on activity. Taken together, these nucleases can show significant off-target effects but it can be challenging to predict these sites. Described herein are methods for increasing the specificity of genome editing using the CRISPR/Cas system, e.g., using RNA-guided Foki Nucleases (RFNs), e.g., Fokl-Cas9 or Foki-dCas9-based fusion proteins.

Find Patent Forward Citations

Joung, J.. (2018). Using rna-guided foki nucleases (rfns) to increase specificity for rna-guided genome editing (U.S. Patent No. 10011850). U.S. Patent and Trademark Office. https://idiyas.com/patent/badge/10011850

Data source: USPTO (United States Patent and Trademark Office) public patent records. Weekly synchronization. How IDiyas builds this profile

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