The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jul. 03, 2018
Filed:
Sep. 22, 2015
Applicant:
China Agriculture University, Beijing, CN;
Inventors:
Jianhui Tian, Beijing, CN;
Zhenwei Jia, Beijing, CN;
Jiaxin Zhang, Beijing, CN;
Lei An, Beijing, CN;
Zhonghong Wu, Beijing, CN;
Assignee:
China Agriculture University, Beijing, CN;
Attorney:
Primary Examiner:
Assistant Examiner:
Int. Cl.
CPC ...
C12N 5/075 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0609 (2013.01); C12N 2501/11 (2013.01); C12N 2501/31 (2013.01); C12N 2501/998 (2013.01); C12N 2517/10 (2013.01);
Abstract
Methods are provided for in vitro maturation (IVM) of bovine oocytes that include steps of (a) pre-culturing bovine germinal vesicle (GV) oocytes in the presence of C-type natriuretic peptide (CNP) and (b) subsequently culturing the oocytes of (a) for an extended duration in medium containing follicle stimulating hormone (FSH), luteinizing hormone (LH), 17β-estradiol (E2), epidermal growth factor (EGF), and fetal bovine serum (FBS).