The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 26, 2018

Filed:

Aug. 19, 2013
Applicant:

Kaneka Corporation, Kita-ku, JP;

Inventor:

Shuhei Taniguchi, Settsu, JP;

Assignee:

KANEKA CORPORATION, Kita-ku, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 1/40 (2006.01); B01D 69/02 (2006.01); B01D 69/08 (2006.01); C12N 1/04 (2006.01); C12M 1/00 (2006.01); B01D 71/26 (2006.01); B01D 71/18 (2006.01); B01D 71/68 (2006.01); B01D 71/10 (2006.01); B01D 63/02 (2006.01); C12M 1/12 (2006.01); B01D 61/18 (2006.01);
U.S. Cl.
CPC ...
G01N 1/4077 (2013.01); B01D 61/18 (2013.01); B01D 63/02 (2013.01); B01D 69/02 (2013.01); B01D 69/081 (2013.01); B01D 71/10 (2013.01); B01D 71/26 (2013.01); B01D 71/68 (2013.01); C12M 47/02 (2013.01); C12N 1/04 (2013.01); C12M 25/10 (2013.01); G01N 2001/4088 (2013.01);
Abstract

Provided is a simple method for producing a cell concentrate (other than human blood) in a short time with neither loss of cells nor an excessive burden on cells. Included is a method for producing a cell concentrate using an inside-out filtration system for processing a cell suspension, the system including: a cell suspension inlet port; a filtrate outlet port; a cell suspension outlet port; and hollow fiber separation membranes provided between the cell suspension inlet port and the cell suspension outlet port, wherein the membranes have a total cross-sectional area of 0.5-1.5 cm, the membranes have an inside membrane area of 0.2 mor less, the suspension is flowed inside the membranes at a linear velocity of 500-1200 cm/min, and the quotient of the division of the initial filtrate flow rate from the filtrate outlet port by the flow rate into the cell suspension inlet port is 0.4-0.7.


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