The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 26, 2018

Filed:

Mar. 27, 2013
Applicant:

Variation Biotechnologies, Inc., Gatineau, CA;

Inventors:

David E. Anderson, Boston, MA (US);

Jasminka Bozic, Ottawa, CA;

Barthelemy Ontsouka, Montreal, CA;

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12Q 1/70 (2006.01); G01N 33/50 (2006.01); G01N 33/68 (2006.01); A61K 39/12 (2006.01); A61K 39/00 (2006.01);
U.S. Cl.
CPC ...
C12Q 1/70 (2013.01); A61K 39/12 (2013.01); G01N 33/5044 (2013.01); G01N 33/6854 (2013.01); A61K 2039/5258 (2013.01); A61K 2039/55505 (2013.01); A61K 2039/70 (2013.01); C12N 2710/16134 (2013.01); G01N 2333/045 (2013.01); G01N 2469/20 (2013.01);
Abstract

The present disclosure provides methods useful for determining levels of HCMV infection in host cells and, by extension, determining levels of neutralizing antibodies present in a sample. The present disclosure encompasses the recognition that HCMV viruses that have a fluorescent moiety permit detection of viral infection (e.g., by assessing fluorescence in cells after contacting the host cell with the virus). In some embodiments, levels of HCMV infection are determined by fluorescence detection where the virus has been preincubated with a test sample (e.g., a serum sample) from a subject. In some embodiments, the subject has been administered a candidate HCMV vaccine.


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