The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 26, 2018

Filed:

May. 20, 2016
Applicant:

The Trustees of the University of Pennsylvania, Philadelphia, PA (US);

Inventors:

Katalin Kariko, Rydal, PA (US);

Drew Weissman, Wynnewood, PA (US);

Gary Dahl, Madison, WI (US);

Anthony Person, Madison, WI (US);

Judith Meis, Fitchburg, WI (US);

Jerome Jendrisak, Madison, WI (US);

Assignee:
Attorneys:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/00 (2006.01); C12N 5/02 (2006.01); C12Q 1/68 (2018.01); C12N 5/074 (2010.01); C12N 15/87 (2006.01); C12N 15/85 (2006.01); A61K 38/17 (2006.01); A61K 38/18 (2006.01); A61K 38/44 (2006.01); A61K 38/46 (2006.01); A61K 38/50 (2006.01); A61K 48/00 (2006.01); C07K 14/47 (2006.01); C07K 14/505 (2006.01); C12N 9/02 (2006.01); C12N 9/22 (2006.01); C12N 9/78 (2006.01); C12N 15/117 (2010.01);
U.S. Cl.
CPC ...
C12N 5/0696 (2013.01); A61K 38/1709 (2013.01); A61K 38/1816 (2013.01); A61K 38/44 (2013.01); A61K 38/465 (2013.01); A61K 38/50 (2013.01); A61K 48/005 (2013.01); A61K 48/0075 (2013.01); C07K 14/47 (2013.01); C07K 14/4712 (2013.01); C07K 14/505 (2013.01); C12N 9/0075 (2013.01); C12N 9/22 (2013.01); C12N 9/78 (2013.01); C12N 15/117 (2013.01); C12N 15/85 (2013.01); C12N 15/87 (2013.01); C12N 2310/17 (2013.01); C12N 2310/335 (2013.01); C12N 2320/30 (2013.01); C12N 2501/602 (2013.01); C12N 2501/603 (2013.01); C12N 2501/604 (2013.01); C12N 2501/605 (2013.01); C12N 2501/606 (2013.01); C12N 2501/608 (2013.01); C12N 2506/02 (2013.01); C12Y 114/13039 (2013.01); C12Y 301/04012 (2013.01); C12Y 305/04004 (2013.01);
Abstract

The present invention provides compositions and methods for reprogramming somatic cells using purified RNA preparations comprising single-strand mRNA encoding an iPS cell induction factor. The purified RNA preparations are preferably substantially free of RNA contaminant molecules that: i) would activate an immune response in the somatic cells, ii) would decrease expression of the single-stranded mRNA in the somatic cells, and/or iii) active RNA sensors in the somatic cells. In certain embodiments, the purified RNA preparations are substantially free of partial mRNAs, double-stranded RNAs, un-capped RNA molecules, and/or single-stranded run-on mRNAs.


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