The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 26, 2018

Filed:

Mar. 16, 2016
Applicant:

Battelle Memorial Institute, Richland, WA (US);

Inventors:

Curtis J. Larimer, Richland, WA (US);

Jonathan D. Suter, Richland, WA (US);

Raymond S. Addleman, Benton City, WA (US);

George T. Bonheyo, Sequim, WA (US);

Michelle R. Brann, Richland, WA (US);

Assignee:

BATTELLE MEMORIAL INSTITUTE, Richland, WA (US);

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12M 1/00 (2006.01); C12M 1/34 (2006.01); C12M 1/32 (2006.01); C12M 1/36 (2006.01); G01B 9/02 (2006.01); G01B 11/06 (2006.01); G01B 11/24 (2006.01);
U.S. Cl.
CPC ...
C12M 41/46 (2013.01); C12M 23/12 (2013.01); C12M 23/22 (2013.01); C12M 23/58 (2013.01); C12M 29/10 (2013.01); C12M 41/36 (2013.01); C12M 41/48 (2013.01); G01B 9/0209 (2013.01); G01B 9/02021 (2013.01); G01B 11/0675 (2013.01); G01B 11/2441 (2013.01);
Abstract

An apparatus and method of measuring biofilm and biological activity on a surface is disclosed. The apparatus includes a biofilm, which includes one or more microorganisms, grown on a substrate. A viewing window is placed on a surface of the biofilm and a gas bubble is introduced between the viewing window and the surface of the biofilm. The space between the substrate and the viewing window may be enclosed in a casing that has an inlet and an outlet, forming a flow cell. A microscope system, such as a white light interferometer, captures data of the biofilm in situ and non-destructively. The 3D images of biofilm surface have high resolution while maintaining a large field of view. The apparatuses and methods will be useful for fundamental studies of biofilms, biomedical and environmental screening, and many other applications in biology and the life sciences.


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