The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.
The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.
Patent No.:
Date of Patent:
Jun. 19, 2018
Filed:
Oct. 07, 2013
Applicant:
Applied Biosystems, Llc, Carlsbad, CA (US);
Inventors:
David C. Woo, Foster City, CA (US);
Clinton Lewis, Palo Alto, CA (US);
Nasser M. Abbasi, San Mateo, CA (US);
Assignee:
APPLIED BIOSYSTEMS, LLC, Carlsbad, CA (US);
Attorney:
Primary Examiner:
Int. Cl.
CPC ...
G01N 33/48 (2006.01); G06F 19/20 (2011.01); C12Q 1/68 (2018.01); G06K 9/00 (2006.01);
U.S. Cl.
CPC ...
G06F 19/20 (2013.01); C12Q 1/6851 (2013.01); G06K 9/00523 (2013.01);
Abstract
The invention discloses a system and methods for quantitating the presence of nucleic acid sequences by evaluation of amplification data generated using real-time PCR. In one aspect, the methods may be adapted to identify a threshold and threshold cycle for one or more reactions based upon evaluation of exponential and baseline regions for each amplification reaction. The methodology used in the analysis may be readily automated such that subjective user interpretation of the data is substantially reduced or eliminated.