The patent badge is an abbreviated version of the USPTO patent document. The patent badge does contain a link to the full patent document.

The patent badge is an abbreviated version of the USPTO patent document. The patent badge covers the following: Patent number, Date patent was issued, Date patent was filed, Title of the patent, Applicant, Inventor, Assignee, Attorney firm, Primary examiner, Assistant examiner, CPCs, and Abstract. The patent badge does contain a link to the full patent document (in Adobe Acrobat format, aka pdf). To download or print any patent click here.

Date of Patent:
Jun. 19, 2018

Filed:

Oct. 20, 2011
Applicants:

Shoen Kume, Kumamoto, JP;

Fumio Endo, Kumamoto, JP;

Nobuaki Shiraki, Kumamoto, JP;

Yasuko Shiraki, Kumamoto, JP;

Kazuhiko Kume, Kumamoto, JP;

Inventors:

Shoen Kume, Kumamoto, JP;

Fumio Endo, Kumamoto, JP;

Nobuaki Shiraki, Kumamoto, JP;

Yasuko Shiraki, Kumamoto, JP;

Kazuhiko Kume, Kumamoto, JP;

Attorney:
Primary Examiner:
Int. Cl.
CPC ...
C12N 5/071 (2010.01);
U.S. Cl.
CPC ...
C12N 5/067 (2013.01); C12N 5/0602 (2013.01); C12N 2500/32 (2013.01); C12N 2506/02 (2013.01); C12N 2506/45 (2013.01); C12N 2533/52 (2013.01);
Abstract

The purpose of the present invention is to provide a method and a culture medium which are capable of efficiently inducing the differentiation of pluripotent stem cells such as ES cells and iPS cells into desired cells by a simple means. The differentiation of pluripotent stem cells can be induced by culturing mammal-derived pluripotent stem cells in a differentiation culture medium that does not contain at least one amino acid selected from the group consisting of methionine, leucine, cysteine, tyrosine and arginine. Also provided is a differentiation-inducing culture medium which does not contain at least one amino acid selected from the group consisting of methionine, leucine, cysteine, tyrosine and arginine.


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