The Royal Institution for the Advancement of Learning / Mcgill University

Universitéde Montreal

Étienne Durette

Michel Bouvier

Stephane Alain Laporte

Mireille Hogue

Christian Le Gouill

Viktoriya Lukasheva

Stéphane Alain Laporte

Yoon Namkung

Hiroyuki Kobayashi

Denis Deblois

Bioluminescence resonance energy transfer (BRET) biosensors for assessing the intracellular localization, internalization and trafficking into cellular compartments of proteins such as receptors, and other biomolecules such as second messengers, are disclosed. These biosensors, which are dependent on the concentration/density of the BRET donor and acceptor in cellular compartments rather that specific protein-protein interactions, use aGFP/Luc BRET pair, which allows the robust and reproducible monitoring of protein trafficking/localization, with a sensitivity compatible with high-throughput screening (HTS). The use of these biosensors for various applications, including assessing/monitoring protein endocytosis, recycling and intracellular trafficking, receptor maturation/rescue by pharmacological chaperones, various endocytosis/exocytosis processes, activation/inhibition, as well as biomolecule concentration/density in different cellular compartments, is also disclosed.

Renilla based biosensors for monitoring biomolecule localization and trafficking in cells

Growing community of inventors

Saint-Jérôme, Canada

Étienne Durette