Other

Fujian Normal University

Zufang Huang

Rong Chen

Yongzeng Li

Shangyuan Feng

Yun Yu

Qiwen Wang

Lan Wang

Guannan Chen

Juqiang Lin

Duo Lin

Min Cheng

Haishan Zeng

Zuci Zheng

Cuncheng Weng

The present invention discloses a detection and analysis method for urine-modified nucleoside based on a surface-enhanced resonance Raman spectroscopy technology. In the method, a tumor marker modified nucleoside in the urine of a normal person and a cancer patient is extracted through the specificity of borophenylic acid gel; Au colloid is taken as an enhancing substrate to detect SERRS signals; and statistical analysis is conducted by using PLS-DA algorithms to establish a diagnosis and identification model for the SERRS of the urine-modified nucleoside. The model is used to discriminate that the to-be-detected urine-modified nucleoside belongs to the normal person or the cancer patient. After PLS-DA, the surface-enhanced resonance Raman spectroscopy data of the urine-modified nucleoside of the present invention has specificity of 96.9%, sensitivity of 98.2% and accuracy of 97.6%. The present invention has the characteristic of rapid and objective detection, and can provide important reference for doctors to diagnose esophagus cancer.

Detection and analysis method for urine-modified nucleoside based on surface-enhanced resonance Raman spectroscopy

This invention provides a body fluid detection method by using surface enhanced Raman spectroscopy. In this method, some biological macromolecules in body fluid samples could be separated with membrane electrophoresis technique firstly. Next, samples are cut off along with the substrates and touched with glacial acetic acid. Transparent colloid formed while incubating. Then add enhancing substrates and continue to incubate and stir. When solid impurities precipitated, stop incubating and stand for layering. In the end, take upper layer resulted to be tested using SERS detection method and build SERS database. This invention successfully eliminated disturbance of other complex components on the SERS detection of protein, DNA and RNA. High quality SERS spectrum obtained is beneficial to the analysis and process of SERS spectrum. Thus body fluid can be differentiated by comparing body fluid SERS spectrum belonging to the healthy people and patients.

Body fluid detection method using surface enhanced Raman spectroscopy

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Fujian, China

Zufang Huang