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Zsofia Banfalvi

Gary Stacey

Anthony J Nieuwkoop

Nirupama A Deshmane

Maria G Schell

Recombinant DNA clones according to the invention contain nodABCDIJL genes of B. japonicum. These clones can be used to practice a method wherein a nod- strain of Rhizobium or Bradyrhizobium bacteria lacking one or more of these genes is converted to a nod+ strain by transfering one or more of the missing genes to the strain. According to a further aspect of the invention, nucleotide sequences have been identified which control the expression of nod LABC genes of Bradyrhizobium japonicum in the presence of flavones produced by the plant roots. By linking such sequences with a gene having a desired characteristic, such as a gene encoding for production of a plant parasite toxin, bacterial can be created which selectively express the characteristic only in the presence of the flavone which triggers expression of the gene.

Recombinant DNA clones of essential nodulation genes of bradyrhizobium

Recombinant DNA clones according to the invention contain a host range gene of B. japonicum which is contained within a 3.3 kb HindIII restriction fragment from the B. japonicum chromosome. Mutations to this gene change the ability of the bacteria to nodulate different plants. In one example, a Tn5 insertion to this region caused the strain to lose its ability to nodulate siratro, but nodulation of soybeans was unaffected. This gene may be manipulated to extend or restrict the host range of the strain for agricultural purposes.

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H-6723 Szeged Vajda, Hungary

Zsofia Banfalvi