Egis Gyogyszergyar Zrt.

Semmelweis Egyetem

Eotvos Lorand Tudomanyegyetem

Hun-ren Természettudományi Kutatóközpont

Zoltán Varga

Krisztina Móricz

Gábor Gigler

Gergely Szakács

András Füredi

Edit Pap

István Gacsályi

Adrienn Pálvölgyi

Attila Bóta

András Ferenc Wacha

Anita Gulyás

Dániel Ulej

Barbara Wernerné Sódar

Edit Irén Buzás

Tamás Visnovitz

Xabier Osteikoetxea Velez

Kristóf Hegedüs

Krisztina Kiss

Lívia Nagyné Naszályi

Anikó Gaál

Szilárd Tóth

Gergö Barta

The field of extracellular vesicles (EVs) currently attracts substantial attention in biomedicine due to the proposed role of EVs in various biological processes and their potential of serving as biomarkers for diseases. However, the availability of approaches for reliable and reproducible standardised quantification of EVs is limited. Therefore, current interest in EV research urges reliable tools of standardization and accurate enumeration of EVs, preferably on the basis of lipid quantification. By definition, EVs are surrounded by phospholipid bilayers, therefore, lipids (such as phospholipids and cholesterol) are essential components of all EVs. The method of the invention avoids the overestimation of EV concentration based on the protein measurement, as it focuses on the defining component of EVs, the lipid bilayer. Our method can be used virtually in any standard laboratories where a fume hood, a thermoblock, and a spectrophotometer are available. The application does not require expensive equipment, therefore it can be an easy, reliable and quick method for quantification of EVs and standardisation of EV experiments.

Method for determining the lipid content of extracellular vesicles

The present invention relates to a topical pharmaceutical composition comprising pregabalin and a reduced micellar phospholipid as an active ingredient, which results in a prolonged analgesic effect of pregabalin. The product can reduce neuropathic pain by at least 5 hours.

Topical pharmaceutical composition containing phospholipids

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Inárcs, Hungary

Zoltán Varga