Agilent Technologies, Inc.

University of Colorado

Zoltan Kupihar

Douglas J Dellinger

Marvin H Caruthers

Geraldine F Dellinger

Joel Myerson

Agnieszka Sierzchala

John Turner

Fernando Ferreira

Zoltan Timar

Aspects of the invention include 2' protected nucleoside monomers that are protected at the 2&#x2032; site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.

Thiocarbon-protecting groups for RNA synthesis

Embodiments of the invention include nucleotide and nucleoside monomers protected at the 5'- or 3&#x2032;-hydroxyls with thioether substituted aryl carbonate protecting groups. In certain cases, the carbonate protecting groups include an aryl moiety, e.g., a phenyl group, attached to the carbonate, where the aryl moiety further includes a thioether group, e.g., an alkyl or aryl thioether group, bound directly to the aryl ring. Aspects of the invention further include methods of synthesizing nucleic acids, e.g., oligonucleotides, using such protected nucleoside monomer monomers, as well as nucleic acids produced using methods of the invention and compositions thereof.

Thioether substituted aryl carbonate protecting groups

Methods of forming polynucleotides are disclosed.

Methods and compounds for polynucleotide synthesis

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Szeged, Hungary

Zoltan Kupihar