Bristol-myers Squibb Compnay

Zizhuo Xing

Paul M Skonezny

Shu-Jen Chiang

Li You

Thomas J Franceschini

Gregory L Stein

Nigel Ian Bowers

Wendy L Anderson

Kin S Lam

Brian A Dougherty

Wenying Li

Sandra J Hofstead

Yijun Chen

Christopher L Steele

The present invention relates to a process for preparing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane comprising contacting a mutagenizedhaving ATCC deposit no. PTA-6648 with (3S)-1-halo-2-oxo-3-(protected)amino-4-substituted butane substrate. The present invention also relates to a process comprising mixing (2R,3S)-1-halo-2-hydroxy-3-(protected)amino-4-substituted butane with at least one base in the presence of at least one solvent to produce a reaction mixture containing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane. The present invention further relates to a process comprising crystallizing the (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane out of the reaction mixture by concurrently adding water and the reaction mixture together. The present invention is also directed to a mutagenizedhaving ATCC deposit no. PTA-6648.

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Process for preparing (2R,3S)-1,2-epoxy-3-(protected)amino-4-substituted butane and intermediates thereof

Isolated nucleic acid and amino acid sequences for phenylalanine aminomutase enzyme and methods for purifying this enzyme are provided. Methods for altering biosynthesis of compounds in plant cell cultures are also provided. In particular, methods for altering production of taxanes and taxane-related compounds are provided.

Compositions and methods for altering biosynthesis of taxanes and taxane-related compounds

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Syracuse, NY, United States of America

Zizhuo Xing