University of Iowa Research Foundation

University of Kansas

University of Missouri

Targeted Genetics Corporation

Ziying Yan

John F Engelhardt

Jianming Qiu

Michael J Welsh

Randall S Prather

Christopher S Rogers

Zekun Wang

Dongsheng Duan

Keith L Munson

John F Englehardt

Randall Prather

Christopher Rogers

A recombinant mutant BoV genome is provided, as well as methods of using the vector, e.g., to prepare helper-free virus.

cis and trans requirements for terminal resolution of human bocavirus 1

Vectors having a nucleotide sequence having SEQ ID NO:1 or a nucleotide sequence having at least 85% identity to SEQ ID NO:1, or a portion thereof, that is capable of regulatingreplication, or vectors having the complement of the nucleotide sequence, and methods of using the vectors, are provided.

small noncoding RNA and uses thereof

A method of preparing a chimeric virus comprising bocavirus capsid protein (VP) and a recombinant adeno-associated (AAV) viral genome, and isolated mutant bocavirus genomes, are provided.

Methods to produce chimeric adeno-associated virus/bocavirus parvovirus

An isolated recombinant parvovirus vector comprising a synthetic enhancer comprising plurality of enhancer sequences operably linked to a promoter, and methods of using the vector, are provided.

AAV-mediated expression using a synthetic promoter and enhancer

Vectors having a nucleotide sequence having SEQ ID NO:1 or a nucleotide sequence having at least 85% identity to SEQ ID NO:1, or a portion thereof, that is capable of regulating bocaparvovirus replication, or vectors having the complement of the nucleotide sequence, and methods of using the vectors, are provided.

Bocaparvovirus small noncoding RNA and uses thereof

The invention provides an isolated chimeric virus comprising bocavirus capsid protein and a recombinant adeno-associated viral (AAV) genome, an isolated rBoV comprising human bocavirus capsid protein and a recombinant BoV genome, and uses therefor. For example, the chimeric virus may be employed to deliver transgenes, such as those encoding therapeutic or prophylactic gene products, to mammalian cells.

Chimeric adeno-associated virus/ bocavirus parvovirus vector

The invention provides an isolated chimeric virus comprising bocavirus capsid protein, e.g., an isolated chimeric virus comprising human bocavirus capsid protein, and a recombinant adeno-associated viral (AAV) genome, an isolated recombinant bocavirus (rBoV) comprising human bocavirus capsid protein and a recombinant Boy genome, and uses therefor, for example, in gene therapy for diseases in a mammal including diseases with aberrant expression of an endogenous gene product.

The present invention provides transgenic, large non-human animal models of diseases and conditions, as well as methods of making and using such animal models in the identification and characterization of therapies for the diseases and conditions.

Transgenic pig model of cystic fibrosis

Transgenic porcine models of cystic fibrosis

The invention provides recombinant AAV vectors, at least one of which has heterologous terminal palindromic sequences, and methods of using those vectors.

Adeno-associated virus vectors with intravector heterologous terminal palindromic sequences

The present invention provides transgenic, large non-human animal models of diseases and conditions, as well as methods of using such animal models in the identification and characterization of therapies for the diseases and conditions.

Method of identifying compounds using a transgenic pig model of cystic fibrosis

Agents and methods to alter rAAV transduction are provided.

Compounds and methods to enhance rAAV transduction

The invention provides an isolated and purified DNA molecule comprising at least one DNA segment, a biologically active subunit or variant thereof, of a circular intermediate of adeno-associated virus, which DNA segment confers increased episomal stability, persistence or abundance of the isolated DNA molecule in a host cell. The invention also provides a composition comprising at least two adeno-associated virus vectors.

Growing community of inventors

Iowa City, IA, United States of America

Ziying Yan