Nanjingjinsirui Science & Technology Biology Corporation

Genscript Holdings (hong Kong) Limited

Pharmaseq, Inc.

Genscript Hongkong

Jinsite Science & Technology (nanjing) Co., Ltd.

Hunan Zonsen Peplib Biotechco., Ltd

Hunan Zonsen Peplib Biotech Co., Ltd

Zhuying Wang

Fang Liang Zhang

Tao Bai

Tao Wang

Wenzhu Chen

Xiaowu Liu

Ping Yang

Weiqiang Liu

Wlodek Mandecki

Hong Qian

Luquan Wang

Zhan Dai

Bing Lai

Ji Li

Fanli Meng

Yun He

Chunjiao Wang

Zhibing Liu

Tianhui Xia

Jinyu Yang

Yuanming Liao

Xiangqun Li

An improved peptide library preparation method is described for constructing complete peptide libraries such as a complete tripeptide library, tetrapeptide library, pentapeptide library, hexapeptide library, heptapeptide library, or a complete octapeptide library, etc. The method includes constructing an expression vector for the expression of tagged peptides. Each tagged peptide contains an array of peptides of different sizes, and the number of peptides in a complete peptide library can be dramatically reduced relative to conventional chemical peptide synthesis. Furthermore, the libraries can be readily reproduced. The improved peptide library preparation method can particularly be used, for example, to construct a complete pentapeptide library. Other related methods and related expression vectors are also described.

Peptide library constructing method and related vectors

A device for gel electrophoresis is provided having larger wells for loading an increased sample volume with improved gel resolution. The device includes a gel cassette having a front plate and a back plate, wherein at least one plate has a stepped inner surface to create a wider opening at the top of the gel cassette, a gel matrix, and a comb with teeth having a thickness substantially equal to the spacing at the top opening of the gel cassette. Using this device, the sample volume in each well can be increased and the sample height in each well can be significantly reduced, as compared to loading the same volume in the wells of a standard gel cassette.

Gel electrophoresis device for loading large sample volumes

An improved staining method is described for staining a biopolymer such as a peptide, a protein, an RNA, a DNA, an oligosaccharide or a complex containing a peptide, a protein, an RNA, a DNA, or an oligosaccharide in a matrix. The method includes the step of moving a staining reagent into the matrix using an electric force. The staining time can be dramatically reduced relative to conventional technologies. The improved staining method can particularly be used, for example, to stain proteins after gel separation. Other related methods and related kits are also described.

Rapid electrophoresis binding method and related kits and compositions

Methods and compositions for cloning a donor DNA molecule into an acceptor vector at a predetermined location are described. The methods are based on homologous recombination mediated by in vitro treatment of the donor DNA and the acceptor vector with an enzyme cocktail containing an exonuclease and a single-stranded DNA binding protein.

Homologous recombination-based DNA cloning methods and compositions

Provided, among other things, is a multiplex assay comprising: conducting a fluorescence-developing assay on microtabs having at least one surface that shows plasmonic enhancement, wherein a plurality of the microtabs have unique probes affixed to their plasmonically enhanced surfaces; and measuring the fluorescence associated with the substrates and identifying the correlated probe by for the microtab. The microtabs can be, for example, MTPs that send a unique identifier, and the correlated probe can be identified by querying the MTPs for their identifier.

Metal nanoparticle structures for enhancing fluorescence-based assays

Optimized signal peptide coding sequences for enhanced expression and secretion of protein from a cell and related compositions and methods are described. The optimized signal peptide coding sequence encodes an mRNA that contains at least one hairpin structure immediately downstream of the initiation codon. Methods for obtaining the optimized signal peptide coding sequences and methods for enhanced expression and secretion of proteins using the optimized signal peptide coding sequences are also described.

Methods and compositions for enhanced expression and secretion of proteins

Homologous recombination-based DNA cloning compositions

The present invention provides improved and rapid detection methods for an antigen such as a chemical compound, a peptide, a nucleic acid, or a protein released from cells or virus particles in situ. The detection time for an antigen can be dramatically reduced relative to conventional technologies. The technology can particularly be used, for example, to modify and reduce the detection time significantly in traditional ELISA, and also Western blot or Dot blot assays. The improved ELISA method is rapid, economical, reproducible, simple and automatable. Also provided are compositions and kits for using the improved ELISA methods for the rapid detection of antigens.

Composition related to rapid ELISA process

An improved gene sequence optimization method, the systematic optimization method, is described for boosting the recombinant expression of genes in bacteria, yeast, insect and mammalian cells. This general method takes into account of multiple, preferably most or all, of the parameters and factors affecting protein expression including codon usage, tRNA usage, GC-content, ribosome binding sequences, promoter, 5'-UTR, ORF and 3&#x2032;-UTR sequences of the genes to improve and optimize the gene sequences to boost the protein expression of the genes in bacteria, yeast, insect and mammalian cells. In particular, the invention relates to a system and a method for sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm. The improved systematic optimization method can be incorporated into a software for more efficient optimization.

Method of sequence optimization for improved recombinant protein expression using a particle swarm optimization algorithm

Rapid ELISA processes and related compositions

Rapid C-ELISA process and related compositions

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Monmouth Junction, NJ, United States of America

Zhuying Wang