Syngenta Participations Ag

Syngenta Crop Protection Ag

Zhongying Chen

Qiudeng Que

Mary-Dell Matchett Chilton

Heng Zhong

Weining Gu

Myoung Kim

Ailing Zhou

Yaping Jiang

Mark Scott Rose

Yinping Lucy Qin

Timothy Kelliher

Joseph Dallas Clarke, V

Jonathan Cohn

Michele Susan Yarnall

Jiang Li

Shujie Dong

Wenling Wang

Yuejin Sun

Nan Zhou

Lucy Qin

Julie Leonard Green

Wan Shi

Guozhu Tang

Mark Rose

Eric Levy

Yingping Lucy Qin

Mary-Dell Chilton

Systems and methods for producing a plurality of unique edits in a plant's T1 seed. In one example, a method comprises transforming at least one expression cassette into a plant cell or a plant tissue. The at least one expression cassette may comprise a nucleic acid that encodes a DNA modification enzyme; optionally, a nucleic acid that encodes at least one guide RNA (gRNA); and a floral mosaic (FMOS) regulatory sequence, wherein the FMOS regulatory sequence (i) mediates expression of the DNA modification enzyme in at least one of a floral primordia cell and a floral reproductive organ, and (ii) mediates a plurality of edits in the at least one of the floral primordia and the floral reproductive organ. The method may also include regenerating the plant cell or plant tissue into a T0 plant having a plurality of T1 seed, wherein the T1 seed contain a plurality of unique edits.

Compositions and methods for driving T1 event diversity

The present invention relates to methods and compositions for chimeric RNA comprising a guide RNA and bait RNA for modifying a target site in the genome of a cell. Such modifications include integration of a transgene and allelic mutations and modifications of native genes. Also provided are plants comprising a modified nucleic acid sequence compared to the native gene integrated into a targeted genomic site in the plant genome.

Methods and compositions for targeted genomic insertion

The present invention relates to methods and compositions for targeted insertion of polynucleotide molecules into ideal target sites in the genome of a maize plant. The present invention relates to maize recombinant molecules comprising heterologous sequences and also to methods of integrating a DNA of interest into a target maize genomic locus in a maize genome. The present invention also relates to regenerated maize plants or plant parts comprising the recombinant molecules and/or a DNA of interest.

Targeted insertion sites in the maize genome

Provided herein are promoters and related compositions and methods of use. Such promoters are useful for expression cassettes for plants, such as soybean. Such expression cassettes are useful, e.g., to drive expression of trait genes in plants, such as soybean. In some aspects, the disclosure provides an expression cassette comprising a promoter, a 5' UTR, and an intron.

Soybean promoters and uses thereof

Provided herein are regulatory elements, such as promoters and terminators, derived or obtained from variousspecies. Such regulatory elements are useful for expression cassettes for plants, such as soybean. Such expression cassettes generally contain a promoter and terminator sequence to control expression of each coding sequence. Provided are promoters and terminators which are useful for expressing insect resistance and herbicide tolerance genes.

Glycine regulatory elements and uses thereof

The present invention relates to methods and compositions for modifying a target site in the genome of a plant cell. Such modifications include integration of a transgene and mutations. The present invention also relates to methods and compositions for identifying and enriching for cells which comprise a modified target site.

Methods and compositions for identifying and enriching for cells comprising site specific genomic modifications

The invention provides three novel disarmed strains ofbacteria useful for the transformation of plants. The invention provides three engineeredChry5 strains or bacterial cells thereof which comprise the Chry5 strain chromosomal background and a disarmed pTiChry5 vector, and methods of using said bacterial strains or cells for transformation of fungal or plant cells, in particular dicot or monocot plant cells, including soybean, maize, wheat, and sugarcane cells. The invention further relates to the transgenic plants created by these methods.

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Research Triangle Park, NC, United States of America

Zhongying Chen