Medimmune Limited

Zhongying Chen

Hong Jin

George William Kemble

Vu Truong-Le

Palani Balu

Erich Hoffmann

Gregory Duke

Luisa Yee

Bin Lu

Harshvardhan Mehta

Eric Tsao

Chiaoyin Kathy Wang

Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. A method of producing a cold adapted (ca) influenza virus that replicates efficiently at, e.g., 25&#xb0; C. (and immunogenic compositions comprising the same) is also provided.

Multi plasmid system for the production of influenza virus

The present invention encompasses methods of producing influenza B viruses in cell culture. The influenza B viruses may have desirable characteristics, such as enhanced replication in eggs and may be used, for example, in vaccines and in methods of treatment to protect against influenza B virus infection.

Influenza B viruses having alterations in the hemagglutinin polypeptide

The technology relates in part to modified influenza viruses useful for vaccine development. Polypeptides, polynucleotides, methods, compositions, and vaccines comprising influenza hemagglutinin and neuraminidase variants are provided.

Swine influenza hemagglutinin variants

Influenza B viruses having alterations in the hemaglutinin polypeptide

Method for producing temperature sensitive influenza A viruses

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Cupertino, CA, United States of America

Zhongying Chen