E.i. Dupont De Nemours and Company

Pioneer Hi-bred International, Inc.

Corteva Agriscience LLC

Greenvenus, LLC

Bayer Sas

Zhongsen Li

Saverio Carl Falco

Michael W Lassner

Zhan-Bin Liu

Huirong Gao

Jayantilal Devabhai Patel

Andrew Mark Cigan

Christopher Jay Scelonge

Chadwick Bruce Koscielny

Lomas Tulsieram

David George Charne

Ferdinand Thoonen

Sergei Svitashev

Yongping Zhang

Wenpin Chen

James Jefferson Smith

Derek Jantz

Jeffrey M Hegstad

Mark D Vogt

Christine B Hazel

Zhan-bin Liu

Anthony D Guida, Jr

Sekhar Boddupalli

Arianne Tremblay

Aiqiu Xing

Joshua K Young

Andrey A Boukharov

Shiv Bahadur Tiwari

Stephen Ezra Schauer

Rio Stamler

Ferdinand Gerard Thoonen

Rebecca E Cahoon

Quinn Qun Zhu

J Antoni Rafalski

Anthony John Kinney

Hajime Sakai

Edgar Benjamin Cahoon

David J Peterson

Guo-Hua Miao

James J English

Yiwen Fang

Peizhong Zheng

Joan Tellefsen Odell

Jinrui Shi

Leszek Aleksander Lyznik

John M Salmeron

Jian-Ming Lee

Sabine Susanne Hantke

Michele Morgante

Xiping Niu

Victor Llaca

Stephane Deschamps

Carl Falco

L Aleksander Lyznik

Mark Cigan

Jyoti R Rout

Eric Aasen

Michael Lassnser

John Salmeron

Terry Thomas

Compositions and methods are provided for genome modification of a target sequence in the genome of a plant or plant cell. The methods and compositions employ a guide RNA/Cas endonuclease system to provide an effective system for modifying or altering target sites within the genome of a plant, plant cell or seed. Also provided are compositions and methods employing a guide polynucleotide/Cas endonuclease system for genome modification of a nucleotide sequence in the genome of a cell or organism, for gene editing, and/or for inserting or deleting a polynucleotide of interest into or from the genome of a cell or organism. Once a genomic target site is identified, a variety of methods can be employed to further modify the target sites such that they contain a variety of polynucleotides of interest. Breeding methods and methods for selecting plants utilizing a two component RNA guide and Cas endonuclease system are also disclosed. Compositions and methods are also provided for editing a nucleotide sequence in the genome of a cell.

Plant genome modification using guide RNA/Cas endonuclease systems and methods of use

The invention provides a compositions and methods for controlling phenotypic traits in plants. Genes of interest are placed under the control of a gene switch to allow inducible control or expression of a gene of interest 'on-demand' by treatment of the plant with a chemical ligand.

Control of phenotype in plants

Methods for producing in a plant a complex transgenic trait locus comprising at least two altered target sequences in a genomic region of interest are disclosed. The methods involve the use of two or more double-strand-break-inducing agents, each of which can cause a double-strand break in a target sequence in the genomic region of interest which results in an alteration in the target sequence. Also disclosed are complex transgenic trait loci in plants. A complex transgenic trait locus comprises at least two altered target sequences that are genetically linked to a polynucleotide of interest. Plants, plant cells, plant parts, and seeds comprising one or more complex transgenic trait loci are also disclosed.

Methods for producing a complex transgenic trait locus

The present application is directed to lettuce plants comprising a mutation in each of at least two different PPO genes, where said mutations reduce the activity of PPO protein compared to a wild type lettuce plant. The present application is also directed to methods for making such lettuce plants.

Lettuce with increased shelf life

Compositions and methods are provided for stacking multiple independent transgenic loci into the genome of a plant. Compositions include plants, seeds or plant cells comprising at least one transgenic target site and at least one genomic locus of interest integrated at different genomic sites within a genomic window. Plant breeding techniques can be employed such that the transgenic target site and the genomic locus of interest can be bred together. In this way, multiple independent transgene integrations can be generated within a genomic window to create a complex trait locus. The complex trait locus is designed such that the transgenic target sites and/or genomic loci of interest can segregate independently of each other, thus providing the benefit of altering a complex trait locus by breeding-in and breeding-away specific elements. Various methods can also be employed to modify the target sites such that they contain a variety of polynucleotides of interest.

Methods and compositions for generating complex trait loci

The disclosure relates to gene expression regulatory sequences, specifically to the promoter of a U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

U6 polymerase III promoter and methods of use

Compositions and methods related to transgenic glyphosate tolerantplants are provided. Specifically, the present invention providesplants having a DP-073496-4 event which imparts tolerance to glyphosate. Theplant harboring the DP-073496-4 event at the recited chromosomal location comprises genomic/transgene junctions within SEQ ID NO: 2 or with genomic/transgene junctions as set forth in SEQ ID NO: 12 and/or 13. The characterization of the genomic insertion site of the event provides for an enhanced breeding efficiency and enables the use of molecular markers to track the transgene insert in the breeding populations and progeny thereof. Various methods and compositions for the identification, detection, and use of the event are provided.

Brassica GAT event and compositions and methods for the identification and/or detection thereof

The disclosure relates to gene expression regulatory sequences from maize, specifically to the promoter of a maize U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

The disclosure relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean U6 polymerase III gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, mutated plants, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same.

Soybean U6 polymerase III promoter and methods of use

Growing community of inventors

Hockessin, DE, United States of America

Zhongsen Li