Genentech, Inc.

Genetech, Inc.

F. Hoffmann-la Roche Ag

Zhonghua Lin

Patrick J Lupardus

Gerald R Nakamura

Nancy Chiang

Mercedesz Balazs

Henry Chiu

Ning Chai

Min Xu

Hyunjoo Park

Lee R Swem

Dhaya Seshasayee

Isidro Hotzel

Meredith C Hazen

Yi-Chun Hsiao

Benny Chih

Lee Swern

David Verne Hansen

Ainhoa Echeverria Larraza

Zhaoyu Jin

Juan Zhang

Wyne P Lee

The instant technology generally relates to improved methods for producing antibodies, antibody libraries, hybridomas, hybridoma libraries, etc. For example, these methods increase the number of antigen-specific B cells produced, increase the number of hybridomas, and/or increase the number of monoclonal antibodies that can be made in a given production cycle.

Developing an efficient hybridoma platform for therapeutic antibody discovery

This application discloses, inter alia, certain antibodies that specifically bind to human TREM2 (triggering receptor expressed on myeloid cells 2). In some embodiments, the antibodies act as TREM2 agonists. In some embodiments, antibodies herein specifically bind to the stalk region of intact, human TREM2, without binding to soluble TREM2, which is the product of TREM2 cleavage between residues H157 and S158. In some embodiments, the antibodies act as TREM2 agonists, specifically bind to the stalk region of TREM2 with dissociation constants ranging from 10 nM to as low as 100-500 pM, 10-50 pM, or 1-10 pM, specifically bind to a TREM2 epitope spanning the H157-S158 cleavage site, and do not bind to soluble TREM2. In some embodiments, antibodies herein also inhibit shedding of soluble TREM2 in a human microglia cell model and in vivo in a mouse model, decrease levels of soluble TREM2 in plasma, CSF and/or the brain, enhance survival of human microglia cells, and increase Aβ plaque formation and compaction in a human microglia model (for example, as measured by increased Aβ plaque intensity and/or increased X04 plaque intensity in human microglia), and thus, may provide a number of neuroprotective activities.

Agonists of TREM2

The present invention provides anti-hemagglutinin antibodies, compositions comprising anti-hemagglutinin antibodies, and methods of using the same.

Anti-hemagglutinin antibodies and methods of use

The present invention provides anti-influenza B virus hemagglutinin antibodies, compositions comprising anti-influenza B virus hemagglutinin antibodies, and methods of using the same.

Anti-influenza B virus hemagglutinin antibodies and methods of use

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San Francisco, CA, United States of America

Zhonghua Lin