China Agricultural University

China Agriculture University

Zhonghong Wu

Lei An

Jianhui Tian

Shumin Wang

Jiaxin Zhang

Zhenwei Jia

Weibin Zeng

Methods are provided for in vitro maturation (IVM) of bovine oocytes that include steps of (a) pre-culturing bovine germinal vesicle (GV) oocytes in the presence of C-type natriuretic peptide (CNP) and (b) subsequently culturing the oocytes of (a) for an extended duration in medium containing follicle stimulating hormone (FSH), luteinizing hormone (LH), 17&#x3b2;-estradiol (E2), epidermal growth factor (EGF), and fetal bovine serum (FBS).

Method for in vitro oocyte maturation

The present invention provides a method for inducing twin calving. The method according to the present invention comprises firstly treatment to inducing atresia of the antral follicles on ovary so as to initiate the development of new follicular wave, inducing the development of codominant follicles and further achieving double ovulations by exactly controlling the dosage and time points of hormone injections, and performing artificial insemination to induce the cow to deliver twin calves. The method according to the present invention has relative independent on the technical skills, and a person skilled in the art or a common worker can practice it according to specified operation methods; and during practical application, it does not require special equipment, thus saving cost, as well as improving the social benefit and economic benefit of cow breeding.

Method for inducing twin calving

Growing community of inventors

Beijing, China

Zhonghong Wu