Shanghai Techwell Biopharmaceutical Co., Ltd.

Zhonghao Zhuo

Xiaoming Ji

Xiaoliang Gao

Zhijun Tang

Shidong Liu

Zhaoli Zhang

Bingming He

Yunman Zheng

Yan Li

Jing Xu

Yi Chen

Jun Zhu

Tianhui Xu

Huichun Yang

Jushang Zheng

Mingquan Mei

Yong Yao

Ming Li

A purification method of the compound represented by formula 1 is provided, which includes the following steps: (1) loading crude compound 1 on macroporous adsorbent resin, (2) washing the macroporous adsorbent resin with an aqueous solution, an organic solvent or a mixture solution of organic solvent and water, (3) eluting with an aqueous solution, an organic solvent or a mixture solution of organic solvent and water. The purified compound represented by formula 1 is obtained. 

Purification method of azacyclohexapeptide or its salt

The present invention discloses a crystal of a peptide substance and the preparation method as well as the use thereof. Said crystal B possesses peaks at the following 2&#x3b8; angles in the X-ray diffraction pattern (XRPD): 3.2&#xb1;0.2&#xb0;, 5.4&#xb1;0.2&#xb0;, 6.2&#xb1;0.2&#xb0;, and 9.3&#xb1;0.2&#xb0;.

Crystal of peptide substance as well as the preparation method and use thereof

A process for purifying cyclic lipopeptide compounds or salts thereof comprising the steps of: (1) charging a crude compound of Formula I onto a macroporous adsorption resin; (2) washing the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as a washing liquid; and (3) eluting the compound of Formula I from the macroporous adsorption resin using water, an organic solvent or a mixed solution of an organic solvent and water as an eluent. The purification method has the advantages of using a small amount of organic solvents, using no silica gel, and causing little damage to the environment; the purity of the collected compound of formula I is also improved as compared with the methods previously disclosed. 

Process for purifying cyclolipopeptide compounds or the salts thereof

A method for purifying a cyclic lipopeptide or a salt thereof is provided. The method comprises the steps: (1) extracting a fermentation broth containing a compound of formula I or a salt thereof, to obtain an extract 1 after filtration or centrifugation; (2) diluting or concentrating the extract 1 under vacuum to decrease the content of the organic solvent, to obtain an extract 2; (3) loading the extract 2 onto a macroporous absorption resin; (4) washing the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution; and (5) eluting the compound of formula I off from the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution as an eluant. Compared with the prior art, the purification method has the advantages that fewer organic solvent is used, no silica gel is used, the harm to the environment is less, and the purity of the collected compound of formula I is improved. 

The present invention relates to a lubiprostone crystal, the method for the preparation thereof, and a pharmaceutical composition or kit comprising the same, as well as the use of said crystal in the preparation of a medicament for the treatment of gastrointestinal tract diseases, especially constipation. The X-ray powder diffraction pattern of said crystal comprises characteristic peaks measured at the following 2&#x3b8; reflection angles: 14.6&#xb1;0.2&#xb0;, 17.0&#xb1;0.2&#xb0; and 19.6&#xb1;0.2&#xb0;. As compared to amorphous lubiprostone, the crystal of the present invention has the advantages of relative high purity, stable properties and easy-for-storage and use.

Lubiprostone crystal, the use and the method for the preparation thereof

The crystalline form A of Bimatoprost of formula I, its preparation method and use are provided. There are characteristic peaks where diffraction angles 2&#x3b8; are 3.2&#xb1;0.2&#xb0;, 5.5&#xb1;0.2&#xb0;, 11.4&#xb1;0.2&#xb0;, 16.7&#xb1;0.2&#xb0;, 17.6&#xb1;0.2&#xb0;, 19.9&#xb1;0.2&#xb0;, 20.8&#xb1;02&#xb0; and 22.8&#xb1;0.2&#xb0; in the X-ray powder diffraction pattern of the crystalline form A.

Crystalline form of Bimatoprost, preparation method and use thereof

The invention provides a novel microorganism producing pravastatin sodium, as well as the method for producing pravastatin sodium by using this microorganism.CGMCC 0624 of the invention is highly tolerant to mevastatin sodium, and has a high transformation rate of mevastatin sodium, and can produce pravastatin sodium with a high efficiency and low cost.

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Shanghai, China

Zhonghao Zhuo