California Institute of Technology

Roche Diagnostics Operations, Inc.

Roche Diagnostics Gmbh

Maxygen, Inc.

F. Hoffmann-la Roche Ag

Zhixin Shao

Frances H Arnold

Rainer Schmuck

Wolfgang-Reinhold Knappe

Lorraine Joan Giver

Peter Kratzsch

Huimin H Zhao

Alexander Volkov

Joachim Hoenes

Carina Horn

Joseph A Affholter

Detlef Thym

Daniela Beck

Thomas Meier

Bernd Schneidinger

Thomas Link

Katrin Greppmair

Harald Weisser

Janet Kenklies

Daniela Bunk

The invention provides methods for evolving a polynucleotide toward acquisition of a desired property. Such methods entail incubating a population of parental polynucleotide variants under conditions to generate annealed polynucleotides comprising heteroduplexes. The heteroduplexes are then exposed to a cellular DNA repair system to convert the heteroduplexes to parental polynucleotide variants or recombined polynucleotide variants. The resulting polynucleotides are then screened or selected for the desired property.

Method for creating polynucleotide and polypeptide sequences

The present invention relates to improved variants of soluble pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenases (s-GDH), to genes encoding mutated s-GDH, to mutant proteins of s-GDH with improved substrate specificity for glucose, and to different applications of these s-GDH variants, particularly for determining concentrations of sugar, especially of glucose in a sample.

Forms of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase

The present invention relates to oxidoreductase apoenzyme variants which are enzymatically inactive but have coenzyme-binding properties. Further, the present invention relates to DNA sequences encoding these oxidoreductase apoenzyme variants, expression vectors containing such DNA sequences and the use of these oxidoreductase apoenzyme variants in diagnostic applications.

Inactivated enzyme variants and associated process and reagent system

The present invention relates to mutated genes encoding variants of an-type creatinase (EC 3.5.3.3, alternative name: creatine amidinohydrolase), to the creatinase variants encoded by these genes, and to different applications of these variants of creatinase, particularly to their use for determining the creatinine and/or creatine concentration in a sample.

Variants of Erwinia-type creatinase

A conjugate of a tissue non-specific alkaline phosphatase (tns-AP) and dextran which can be obtained by reacting unglycosylated tns-AP with activated dextran by incubation in aqueous solution, stopping the reaction and isolating the conjugate from the solution. The conjugate obtained in this manner is suitable as a standard for the determination of alkaline phosphatase.

Conjugate of a tissue non-specific alkaline phosphatase and dextran, process for its production and use thereof

A method for in vitro mutagenesis and recombination of polynucleotide sequences based on polymerase-catalyzed extension of primer oligonucleotides is disclosed. The method involves priming template polynucleotide(s) with random-sequences or defined-sequence primers to generate a pool of short DNA fragments with a low level of point mutations. The DNA fragments are subjected to denaturization followed by annealing and further enzyme-catalyzed DNA polymerization. This procedure is repeated a sufficient number of times to produce full-length genes which comprise mutants of the original template polynucleotides. These genes can be further amplified by the polymerase chain reaction and cloned into a vector for expression of the encoded proteins.

ECB deacylase mutants

Recombination of polynucleotide sequences using random or defined primers

Growing community of inventors

Penzberg, Germany

Zhixin Shao