Duke University

Zhixia Ye

Michael David Lynch

Ashley Trahan

Ahmet Bozdag

Daniel Rodriguez

Charles Cooper

Ashley Delanie Trahan

Charlie Bridwell Cooper

Charles Bridwell Cooper

The present disclosure provides compositions and methods for rapid production of chemicals in genetically engineered microorganisms in a large scale. Also provided herein is a high-throughput metabolic engineering platform enabling the rapid optimization of microbial production strains. The platform, which bridges a gap between current in vivo and in vitro bio-production approaches, relies on dynamic minimization of the active metabolic network.

Compositions and methods for metabolic control of a biofermentation process with synthetic metabolic valves

Compositions and methods for robust dynamic metabolic control of 3-hydroxypropionic acid production

Compositions and methods for robust dynamic metabolic control of mevalonate production

Compositions and methods for robust dynamic metabolic control of a biofermentation process

Compositions and methods for screening microorganisms for robust dynamic metabolic control

The present disclosure is related to genetically engineered microbial strains and related bioprocesses for the production of pyruvate and related products. Specifically, the use of dynamically controlled synthetic metabolic valves to reduce the activity of enzymes known to contribute to pyruvate synthesis, leads to increased pyruvate production in a two-stage process rather than a decrease in production.

Compositions and methods for the production of pyruvic acid and related products using dynamic metabolic control

Compositions and methods for robust dynamic metabolic control of alanine production

This invention relates to metabolically engineered microorganisms, such as bacterial and or fungal strains, and bioprocesses utilizing such strains. These strains enable the dynamic control of metabolic pathways, which can be used to optimize production. Dynamic control over metabolism is accomplished via a combination of methodologies including but not limited to transcriptional silencing and controlled enzyme proteolysis. These microbial strains are utilized in a multi-stage bioprocess encompassing at least two stages, the first stage in which microorganisms are grown and metabolism can be optimized for microbial growth and at least one other stage in which growth can be slowed or stopped, and dynamic changes can be made to metabolism to improve the production of desired product, such as a chemical or fuel.

Compositions and methods for rapid and dynamic flux control using synthetic metabolic valves

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Raleigh, NC, United States of America

Zhixia Ye