Agency for Science, Technology and Research

Nanyang Technological University

The Methodist Hospital

Zhiping Wang

Ruige Wu

Wei Ling Wang

Huanming Xia

Joseph Sylvester Chang

Sai Kiang Lim

Lei Zhang

Hong Xie

Jun Wei

Stephen T C Wong

Paul Bigliardi

Mei Bigliardi-Qi

Pin Chuan Chen

Massimo Alberti

Bo Wu

Sriram Gopu

Kemi Cui

Srinivas Ramasamy

Yuri Hebert Dancik

Geok Soon Lim

Yen Peng Daphne Seah

The present disclosure describes a microfluidic chip for culturing and in vitro testing of 3D organotypic cultures. The tests may be performed directly on the organotypic culture in the microfluidic chip. The microfluidic chip includes at least one microfluidic unit which includes two fluidic compartments, such as upper and lower, separated by a permeable supporting structure, one or more access opening for the fluidic compartments, and a set of lids interchangeable with a set of insets. The permeable support structure serves as a support for the organotypic culture. The upper and lower compartments may include inlets and outlets which allow fluids to be perfused into the lower compartment and fluids to be perfused into the upper compartment. The access opening may be closed with a lid or accommodate an inset.

Integrated microfluidic system for culturing and testing

A testing device is provided. The testing device includes a capturing tool and a microfluidic chip having a plurality of chambers connected in a network, a sample receiving port connected to the network, and a guide structure configured to receive the capturing tool, wherein the capturing tool is configured to capture sample in a distal position from the guide structure and further configured to transfer the captured sample to the sample receiving port in a proximal position from the guide structure.

Testing device, microfluidic chip and nucleic acid testing method

According to embodiments of the present invention, a microfluidic device for gel electrophoresis is provided. The microfluidic device includes a sample channel configured to receive a sample; a stacking channel comprising a preloaded stacking reagent; and a separation channel comprising a preloaded separation reagent, wherein the preloaded stacking reagent has a physical characteristic different from that of the preloaded separation reagent; and wherein the sample channel, the stacking channel and the separation channel are in fluid communication with one another. According to further embodiments of the present invention, a method of manufacturing a microfluidic device for gel electrophoresis is also provided.

Microfluidic device for gel electrophoresis and method of manufacturing thereof

A device for extracting at least one analyte may include: a sample reservoir configured to contain a sample comprising at least one target analyte and interfering materials; at least one extraction chamber connected to the sample reservoir; at least one porous structure lining one or more sides of the at least one extraction chamber; and a voltage source configured to provide a first voltage and a second voltage, wherein, when the first voltage is provided, the at least one target analyte and the interfering materials move towards the at least one extraction chamber or to a predetermined area from the at least one extraction chamber, wherein, when the second voltage is provided, the interfering materials pass through and exit the at least one extraction chamber, and the at least one target analyte is stopped from exiting the at least one extraction chamber by means of the at least one porous structure.

Devices for extracting at least one analyte

A microfluidic mixing device for mixing at least two fluids to form a mixed fluid comprising; a first mixing chamber () for receiving the fluids from at least two fluid paths (); a mixing zone () upstream from the mixing chamber () having a first and second fluid path; said first and second fluid paths overlapping at first and second discreet points so as to provide mutual fluid communication between the first and second paths at said discreet points.

Microfluidic mixing apparatus and method

According to various embodiments, a microfluidic agitator device may be provided. The microfluidic agitator device may include: an air inlet; an air outlet; an elastic diaphragm provided between the air inlet and the air outlet and configured to oscillate if an airflow from the air inlet to the air outlet is provided; and a chamber coupled to the elastic diaphragm.

Microfluidic agitator devices and methods for agitation of a fluid

A microfluidic droplet generator device, a kit of parts for assembling a microfluidic droplet generator device, and a method of generating microfluidic droplets. The generator device comprises a substrate; a microfluidic channel formed in the substrate; a fluid outlet in fluid communication with the microfluidic channel; and a mechanical element configured such that vibration of the mechanical element causes droplet dispensing from the fluid outlet.

Microfluidic droplet generator

A method of bonding two components by depositing an amorphous and non-hydrogenated intermediate layer () on one of the components () and arranging the components () in spaced relationship with the intermediate layer () therebetween. The method further comprises heating one or both of the components () before bringing the components () into contact. Finally, a voltage is applied to the components () to create a permanent bond between the two components.

Method of wafer/substrate bonding

Growing community of inventors

Singapore, Singapore

Zhiping Wang