Xiamen Oamic Biotechnology Co., Ltd.

Zhimin Deng

Chenguang Xing

Xijing Zhao

Wei Liu

Gang Liu

Mingtao Zhao

Pu Zheng

Yarou Zeng

Huanyan Lan

Pengcheng Chen

The present disclosure discloses a method for preparing vanillin by fermentation with eugenol as a substrate, preparing vanillin by a mixed fermentation ofOMK-68 andsp. OMK-69, its potency reached 35 g/L, the mass conversion rate of eugenol reaches 83.3%.OMK-68 in the present disclosure can use eugenol as a substrate for fermentation to prepare coniferyl alcohol, and its fermentation effect and substrate utilization rate are far better than wild-typeThesp. OMK-69 in the present disclosure can use coniferyl alcohol as a substrate for fermentation to prepare vanillin, and its fermentation effect and substrate utilization rate are far better than wild-typesp.

Method for preparing vanillin by fermentation with eugenol as substrate

The present invention provides a bacterium and a method for the biological production of Heliotropin by the fermemtation of safrole. In one aspect of the present invention, a process for the conversion of safrole to Heliotropin is achieved by the use of a bacterial strain ofZMT-1 (CCTCC M 2016170). The production method comprises the steps of seeding theculture in the presence of oxygen for 24-36 hours, transforming the safrole substrate for 24-48 hours with 0.5-3 g/L substrate concentration, and reaching the Heliotropin concentration of 160-524 mg/L. The present invention reports, for the first time, on a method for producing the high concentration of Heliotropin by using theZMT-1 strain or the enzyme extracted from the strain.

Strain ofand a method of producing heliotropin with the same strain

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Fujian, China

Zhimin Deng