Reg Life Sciences, LLC

Genomatica, Inc.

Kosan Biosciences Incorporated

Ls9, Inc.

Suzhou Biosynthetica Co., Ltd

Zhihao Hu

Vikranth Arlagadda

Andreas W Schirmer

Elizabeth J Clarke

Eli S Groban

Louis G Hom

Derek L Greenfield

Baolong Zhu

Fernando Valle

Murtaza F Alibhai

Ralph C Reid

Stephen B Del Cardayre

Haibo Wang

Na Trinh

Xuezhi Li

Sungwon Lee

Robert G McDaniel

Noah Helman

George M Church

David Arthur Berry

Jay D Keasling

Shane A Brubaker

Emanuela E Popova

Cindy Chang

Kevin Holden

Lisa C Friedman

Alma Itzel Ramos-Solis

Andreas Rascher

Richard C Hutchinson

Chris Somerville

Grace J Lee

Daniel V Santi

Leonard Katz

Fernando A Sanchez-Riera

Tarah S Baron

Bernardo M Da Costa

John R Haliburton

Junying Fan

C Richard Hutchinson

Scott Allen Frykman

Hugo Gramajo

Junjun Jiang

Feng Tian

Xintong Wang

Kailin Zhang

Kseniya Zakharyevich

Fernando A Sanchez-riera

Elizabeth Clarke

Junyin Fan

Stephen B Del Cardayré

Chris Sommerville

The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

Methods of producing omega-hydroxylated fatty acid derivatives

The present invention provides a recombinant microorganism for producing citicoline and a method for producing citicoline by using the recombinant microorganism, wherein genes for degradation and utilization of citicoline, choline, and phosphocholine are knocked out, In addition, a pyrimidine nucleoside synthesis pathway is genetically engineered to remove feedback inhibition to the synthesis pathway. A yield of more than 20 g/L of citicoline can be obtained with recombinant strains in a 5-liter fermenter by means of a biological fermentation method, achieving industrial mass production with low citicoline production costs and less pollution; therefore, the method is a simple, environmentally friendly and has a relatively high promotion and application value.

Recombinant microorganism for producing citicoline and method for producing citicoline

The disclosure relates to fatty diols and recombinant microorganisms for producing them. More particularly, the disclosure relates to recombinant microorganisms engineered to produce fatty diols via fermentation. Further encompassed is a process that uses the microorganisms to produce fatty diols from a simple carbon source.

Microbial production of fatty diols

The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, and engineered host cells for producing fatty acids and derivates of fatty acids such as acyl-CoA, terminal olefins, fatty aldehydes, fatty alcohols, alkanes, alkenes, wax esters, ketones and internal olefins through altered expression of the transcription factor, fadR.

Methods and compositions for improved production of fatty acids and derivatives thereof

The invention relates to compositions and methods, including polynucleotide sequences, amino acid sequences, recombinant host cells and recombinant host cell cultures engineered to produce fatty acid derivative compositions comprising fatty acids, fatty alcohols, fatty aldehydes, fatty esters, alkanes, terminal olefins, internal olefins or ketones. The fatty acid derivative composition is produced extracellularly with a higher titer, yield or productivity than the corresponding wild type or non-engineered host cell.

Production of fatty acid derivatives

Genetically engineered cells and microorganisms are provided that produce products from the fatty acid biosynthetic pathway (fatty acid derivatives), as well as methods of their use. The products are particularly useful as biofuels.

Recombinantfor enhanced production of fatty acid derivatives

The disclosure relates to variant carboxylic acid reductase (CAR) enzymes for the improved production of fatty alcohols in recombinant host cells.

Car enzymes and improved production of fatty alcohols

The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.

Methods and compositions related to thioesterase enzymes

Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described.

Methods and compositions for producing fatty alcohols

Compositions and methods for production of fatty alcohols using recombinant microorganisms are provided as well as fatty alcohol compositions produced by such methods.

Production of fatty acids and derivatives thereof

Genetically engineered cells and microorganisms are provided that produce fatty alcohols from the fatty acid biosynthetic pathway, as well as methods of their use.

Enhanced production of fatty acid derivatives

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South San Francisco, CA, United States of America

Zhihao Hu