Chinese Academy of Agricultural Sciences

Beijing Normal University

Shandong Landsee Genetics Co., Ltd.

Zhejiang Peptites Biotech Co., Ltd.

Zhiguo Liu

Kui Li

Yulian Mu

Kui Xu

Yude Li

Jinlong He

Haiying Xing

Qimei Chen

Yinghui Wei

Yang Qiu

Changli Ge

Xiaoyan Lin

Huiping Wang

Long Wang

Xinyou Yu

Julang Li

Xuehui Cai

Xiaohang Chen

The invention relates to a compound or a salt thereof, a method for preparation thereof, and use thereof, wherein the compound has the structure of Formula (1):

Compound or salt thereof and preparation method and application of same

A method for detecting bi-allelic gene edited cells based on CRISPR/Cas12a technology is provided. The method provides crRNA, a nucleotide sequence of which that binds target is SEQ ID NO: 6. The crRNA is transcribed from a transcription template in vitro; the transcription template is a product obtained by annealing a single stranded DNA molecule shown in SEQ ID NO: 10 and a single stranded DNA molecule shown in SEQ ID NO: 13. The method provides crRNA of CD71 gene and constructs a CRISPR/Cas12a system using the same for CRISPR/Cas12a detection, achieving the screening for CRISPR/Cas9 induced CD71 gene bi-allelic gene edited cells. This method is simple, easy, fast, and cost-effective for screening a large number of mutant cells.

Method for detecting bi-allelic gene edited cells based on CRISPR/Cas12a technology

Provided is a double-gene knockout vector system, a method for preparing porcine fibroblasts with both CD163 gene and CD13 gene being knocked-out, prepared porcine fibroblasts, and a method for preparing a gene-edited pig with both CD163 gene and CD13 gene being knocked-out. The vector system of the present disclosure comprises a CD163 gene knockout vector and a CD13 gene knockout vector. The CD163 gene knockout vector comprises a gene editing vector backbone and a DNA fragment ligated to the gene editing vector backbone, with a nucleotide sequence of the DNA fragment being shown in any one of SEQ ID NOs: 1-3. The CD13 gene knockout vector comprises a gene editing vector backbone and a DNA fragment ligated to the gene editing vector backbone, a nucleotide sequence of the DNA fragment being shown in any one of SEQ ID NOs: 4-6.

Method for preparing porcine fibroblasts with both CD163 gene and CD13 gene being knocked-out

An optical device for focusing synchrotron radiation light source is disclosed according to the present invention, so as to improve the uniformity of the light intensity of the emergent light, to increase the divergence the emergent light, and to restrain the synchrotron radiation higher harmonics. An outline generatrix of the optical device is a quadratic curve segment or a combination of a plurality of quadratic curve segments, the opening orientations of which are the same, and the optical device includes: a plurality of capillary bodies made of transparent material, wherein the capillary bodies in a center region have a solid construe; the capillary bodies in a periphery region located outside of the center region have a hollow structure.

Optical device for focusing synchrotron radiation light source

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Beijing, China

Zhiguo Liu