Syngenta Participations Ag

Zhigang Guo

Venkata Krishna Kishore

Joseph Richard Byrum

Libardo Andres Gutierrez-Rojas

Daolong Wang

Libardo Andres Gutierrez Rojas

Joseph Dallas Clarke

Min Li

Andres Gutierrez-Rojas

Joseph Dallas V Clarke

Methods for introgressing an allele of interest of a locus associated with a yield trait intogermplasm are provided. In some embodiments, the methods include providing aplant that contains an allele of interest of a locus associated with a yield trait, wherein the locus associated with the yield trait is identifiable by PCR amplification of anucleic acid with a pair of oligonucleotides primers as disclosed herein, and introgressing the allele of interest intogermplasm that lacks the allele. Also provided are methods for identifyingplants that contain at least one allele associated with improved yield, improved maize plants, eliteplants, biomass produced from improvedplants, isolated and purified genetic markers, and compositions that include an amplification primer pair capable of amplifying anucleic acid to generate amarker amplicon.

Yield traits for maize

Methods for method for increasing genetic gain in a breeding process are provided. Also provided are methods for choosing breeding pairs predicted to produce progeny having desired phenotypes, methods for increasing the likelihood of producing progeny individual having desired phenotypes, methods for generating progeny individual having desired genotypes and/or phenotypes, progeny produced thereby, and cells, seeds, parts, and tissues cultures thereof.

Methods for increasing genetic gain in a breeding population

Provided herein are methods for evaluating associations between candidate genes and a trait of interest in a population. The methods include a combination of genome-wide association analysis and one or more of nested association mapping (NAM), expression QTL analysis (eQTL), and allele epistastic analysis (AEA). Markers are selected or prioritized if they are shown to be positively-correlated with a trait of interest using GWA and a combination of one or both of NAM and eQTL. Also provided are models for evaluating the association between a candidate marker and a trait in a nested population of organisms. These methods include single marker regression and multiple marker regression models. Markers identified using the methods of the invention can be used in marker assisted breeding and selection, as genetic markers for constructing linkage maps, for gene discovery, for identifying genes contributing to a trait of interest, and for generating transgenic organisms having a desired trait.

Method for selecting statistically validated candidate genes

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Research Triangle Park, NC, United States of America

Zhigang Guo