Anaspec, Inc.

Aat Bioquest, Inc.

Molecular Probes, Inc.

Life Technologies Corporation

Becton Dickinson and Company Limited

Molecular Devices Corporation

Affymetrix, Inc.

Invitrogen Corporation

Mds Analytical Technologies (us) Inc.

Molecule Probes, Inc.

Zhenjun Diwu

Yi Tang

Jianheng Zhang

Xiang Guobing

Haitao Guo

Jixiang Liu

Ruogu Peng

Richard P Haugland

Jinfang Liao

Qin Zhao

Kyle R Gee

Wayne Forrest Patton

Guoliang Yi

Qinglin Meng

Timothy Dubrovsky

Zhen Luo

Dieter Klaubert

Thomas H Steinberg

Luke D Lavis

Jianjun He

Barnaby Abrams

Yen-Wen Chen

Martin Kirk

Travis Jennings

Pengfei Dong

Castle Funatake

Jesse J Twu

Kelly J Cassutt

Feng Quan Liu

Wai-Yee Leung

Vladimir Martin

Aleksey Rukavishnikov

Yu-Zhong Zhang

Courtenay Hart Kerndt

Xing Han

Jiaxing Liu

Mirion Schultz

Mahesh K Bhalgat

Lok N Neupane

Deven Patel

Chunmei Wei

Jingfang Liao

Fluorescent water soluble conjugated polymers including polycyclic aromatic comonomers are provided. The conjugated polymers can be linked to an acceptor fluorescent dye. The conjugated polymers find use in conjugates with biological substrates having applications in a variety of applications including methods of analyte detection.

Condensed polycyclic conjugated polymers and their use for biological detection

This disclosure provides cyanine dye compounds having linked hydrogen bond-forming groups that can be used for detecting nucleic acids, particularly for fluorescent staining of DNA, in a biological sample. We found that the simultaneous incorporation of at least two hydrogen bond-forming groups (HBGs) into such a nucleic acid stain can provide for crosslinking interactions between the stain and the target nucleic acid, and lead to a significant increase in the detection sensitivity without substantially increasing undesirable cytotoxicity or mutagenicity properties of the dye compounds. The cyanine dye compounds can have a cyanine structure that connects two particular fused heterocycle ring systems, where at least two HBGs are connected to the core structure through linkers. Also provided are nucleic acid complexes including the cyanine dye compounds, and methods of using the compounds to detect nucleic acids in a biological sample.

Fluorogenic cyanine compounds for detecting nucleic acids

Styryl compounds useful for preparing biological detections probes, and the use of the Styryl compounds for the detection, discrimination and quantification of biological targets.

Styryl phenols, derivatives and their use in methods of analyte detection

The invention provides for polyfluoreno[4,5-cde]oxepine conjugates and their use in methods of analyte detection.

Polyfluoreno[4,5-cde]oxepine conjugates and their use in methods of analyte detection

The present invention is generally directed to fluorescent dyes useful for preparing fluorescent oligonucleotides, and the use of the fluorescent oligonucleotides for the detection, discrimination and quantification of molecular biology targets.

Polycyclic phosphoramidtes and their applications

Polyfluoreno[4,5-cde]oxepine polymers and conjugates thereof

The present disclosure provides fluorogenic compounds useful for preparing fluorescent calcium ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators in methods of detection, discrimination and quantification of metal ions. The subject fluorogenic compounds and fluorescent ion indicators can include a chelating group based on a 2-aminophenoxyethylene glycol 2-aminoethyl ether, N,N,N',N&#x2032;-tetraacetic acid (PEGTA) moiety or precursor thereof where the phenyl group of the PEGTA is substituted with or fused with a fluorophore moiety of interest. The subject methods find use in the detection of intracellular calcium ions. Also provided are kits for use in practicing the subject methods.

Fluorogenic calcium ion indicators and methods of using the same

Fluorescent dyes useful for preparing fluorescent metal ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators for the detection, discrimination and quantification of metal cations are provided.

Carbofluorescein lactone ion indicators and their applications

The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

Site-specific labeling of affinity tags in fusion proteins

Fluorescent dyes useful for preparing fluorescent metal ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators for the detection, discrimination and quantification of metal cations.

Fluorescein lactone ion indicators and their applications

Hydroxamate substituted azaindoline cyanine dyes, conjugates thereof and methods of using the same are provided. The subject cyanine dyes include an azaindoline ring having a hydroxamate substituent. The dyes may further include a reactive group moieties (RGM) and/or a water soluble group. Also provided are conjugates of the subject dyes. Also provided are tandem conjugates including a fluorescent protein capable of energy transfer to the dye. Methods of detecting an analyte in a sample by contacting the sample with a detection reagent are provided. The detection agent may be a dye-conjugate that specifically binds the analyte, or may be a reactive dye which conjugates to the analyte. Also provided are compositions, e.g., kits, etc., incorporating such dyes which facilitate use in such methods.

Hydroxamate substituted azaindoline-cyanine dyes and bioconjugates of the same

Growing community of inventors

Sunnyvale, CA, United States of America

Zhenjun Diwu