Centro De Inmunologia Molecular

Biotech Pharmaceutical Co. Ltd.

Zhenhua He

Xianhong Bai

MeyLen Chea

Zhenhua Yang

Yangliu Cai

Xiao Chen

Kaiheng Xiao

Yuemao Liu

Zhi Bai

Rolando Pérez

Tamara González

Loany Calvo

Miguel Arias

Julio Palacios

Meylen Chea

Rolando Perez

Tamara Gonzalez

Provided is a method for obtaining high-yield, stable expression cell clones from myeloma cell lines in a protein-free culture medium. The method is used for industrial production of a recombinant antibody, and includes three stage: (1) adapting to a protein-free culture medium, statically culturing cells at a low density, and gradually reducing a fat-rich supplement to a chemical culture medium; (2) adapting to a protein-free culture medium; culturing cells at a high density, and using a perfusion fermentation system in a laboratory scale; and (3) screening high-yield, stable expression cell clones from the cells after fermentation ends. The cell clone may be used to produce a humanized anti-NeuGcGM3 14F7 recombinant antibody.

Method for obtaining high-yield, stable expression cell clones and antibody molecules obtained thereby

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Beijing, China

Zhenhua He