Honeywell International Inc.

Zhenhong Sun

Tao Pan

Wendy Wang

Xuanbin Liu

Hang Liao

Leon Xu

Yuan Zheng

Embodiments of the present invention relate to a buffer composition for an integrated nucleic acid amplification and hybridization reaction. The buffer comprise about 50-200 mM of a salt, about 10-30 mM Tris-HCl, about 2-10M Water soluble magnesium salt, about 0.05-1.5% surfactant, about 0.05-0.15 mg/ml stabilizing protein about 50-300 nM of one or more primers, about 20-150 uM of one or more dNTPs, about 5-15% glycerine, about 0.5-1.5% formamide and at least about 5 unit/ml polymerase.

Reaction buffer for microarray

A reactor for the quantitative analysis of target nucleic acids using an evanescent wave detection technique and a method for the quantitative analysis of target nucleic acids are provided. The reactor includes a substrate with a cavity, a buffer layer arranged over the substrate, a quartz cover plate arranged over the buffer layer, and inlet and outlet ports. The reactor is thermally and chemically stable for PCR processing and suitable for an evanescent wave detection technique.

Method to increase detection efficiency of real time PCR microarray by quartz material

A reactor for the quantitative analysis of target nucleic acids using an evanescent wave detection technique and a method of use thereof is provided. The reactor includes a substrate with a cavity, a buffer layer arranged over the substrate; a cover plate arranged over the buffer layer, and inlet and outlet ports. The reactor is thermally and chemically stable for PCR processing and suitable for an evanescent wave detection technique.

Reactor for the quantitative analysis of nucleic acids

A programmable probe design of DNA micro array and detection methodology is provided. DNA probes, which are complemented with the target DNA, are designed and classified into groups according to optimum hybridization temperature. The probes are arrayed by the group and immobilized on the substrate surface of the DNA micro array. The control system, imaging system and temperature control system are programmed to cooperate with each other during the detection process. This design increases the detection capabilities of the parallel-analysis system.

Programmable oligonucleotide micro array

The disclosure relates to a microarray reader that includes a light source which emits light and beam shaping elements positioned near the light source to direct the light. The microarray reader further includes a microarray that is at least formed of an optical substrate and a reaction chamber in contact with the optical substrate. A buffer solution is encapsulated by the optical substrate and the reaction chamber and a holder aligns the microarray relative to the light source such that when light hits the buffer solution molecules in the buffer solution are excited to emit fluorescent light. A temperature control component is thermally engaged with the holder such that the temperature control component adjusts the temperature of the buffer solution. The microarray reader further includes a sensor that receives the fluorescent light emitted by the excited molecules in the buffer solution to generate a signal.

Microarray reader based on evanescent wave detection

A method and apparatus for real-time, simultaneous, qualitative measurement of one or more single nucleotide polymorphisms in one or more target nucleic acids is provided. This method involves combining a polymerase chain reaction (PCR) technique with an evanescent wave technique.

Method for single nucleotide polymorphism and mutation detection using real time polymerase chain reaction microarray

A microarray reader () comprises a light source (), beam shaping elements () positioned near the light source (), a moving stage () supporting one or more of the light source () and beam shaping elements (), an optical substrate () supporting an immobilized microarray, a reaction chamber () in contact with the optical substrate () and encapsulating buffer solution, a heating/cooling component () in contact with the reaction chamber (), a synchronization circuit, an optical filter () and an imaging sensor () positioned near the optical filter ().

Microarray reader based on evanescent wave detection and method of reading a microarray

A method and apparatus for real-time, simultaneous, quantitative measurement for detecting a single nucleotide polymorphism in a target nucleic acid is provided. This method involves combining a polymerase chain reaction (PCR) technique with invader assay technique.

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Shanghai, China

Zhenhong Sun