Pioneer Hi-bred International, Inc.

The Goodyear & Tire Rubber Company

Danisco US Inc.

Zhenglin Hou

Jingtong Hou

Phillip A Patten

Takashi Yamamoto

Ruth Cong

Lu Liu

Joshua K Young

Ingrid Udranszky

Virginijus Siksnys

Tautvydas Karvelis

Andre R Abad

Eric J Schepers

Genhai Zhu

Jennifer Kara Barry

Ute Schellenberger

Weiping Xie

James J English

Thomas Chad Wolfe

Jimei Wang

Jun-Zhi Wei

Lan Zhou

Kevin A Fengler

Brad Poland

Jeffrey Sopa

Linda A Castle

Sandeep Kumar

Bailin Li

Derek H Wells

Yumin Tao

Ericka Bermudez

Robert B Meeley

Huirong Gao

Daniel Siehl

Kevin Hayes

Jian Lu

Christopher Lee Rife

Mary Beatty

Gina Marie Zastrow-Hayes

April L Leonard

Girma M Tabor

Henrik Albert

Yuxia Dong

Giedrius Gasiunas

Matthew J Heckert

David J Meyer

Kishore Nannapaneni

April Lynn Leonard

Eric Jude Schepers

Jeffrey Scott Sopa

Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins.

CRISPR-Cas systems for genome editing

CRISPR-cas systems for genome editing

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Insecticidal proteins from plants and methods for their use

Methods and compositions for modulating plant meristems. Methods are provided for modulating fea3 and/or Fcp1 genomic locus in a host plant or plant cell to improve agronomic characteristics such as kernel number.

Methods and compositions to increase yield through modifications of FEA3 genomic locus and associated ligands

Novel engineered non-natural deaminases are provided. Methods and compositions are provided for modifying the genome of a cell, including a plant cell. Genome modification through guided CRISPR polypeptides and multiple deaminases are provided. Engineered non-natural novel deaminases, including adenosine deaminases are provided. These novel deaminases have improved activity in cells including plant cells.

Engineered deaminases

Compositions and methods are provided for novel Cas9 orthologs, including, but not limiting to, novel guide polynucleotide/Cas9 endonucleases complexes, single or dual guide RNAs, guide RNA elements, and Cas9 endonucleases. The present disclosure also describes methods for creating a double strand break in a target polynucleotide, methods for genome modification of a target sequence under various in vivo and in vitro conditions, in the genome of a cell, for gene editing, and for inserting a polynucleotide of interest into the genome of a cell. Also provided are nucleic acid constructs and cells having a modified target site or altered polynucleotide of interest produced by the methods described herein.

Cas9 orthologs

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest, as probes for the isolation of other homologous (or partially homologous) genes. The insecticidal proteins find use in controlling, inhibiting growth or killing lepidopteran, coleopteran, dipteran, fungal, hemipteran, and nematode pest populations and for producing compositions with insecticidal activity.

PHI-4 polypeptides and methods for their use

Compositions and methods for generating maize plants that exhibit resistance to northern leaf blight are provided herein. Isolated polynucleotides encoding a polypeptide that confers resistance to northern leaf blight, polynucleotide constructs comprising such, and maize plants comprising the polynucleotide constructs are provided. The methods include expressing an isolated polynucleotide in a maize cell via standard transformation methods and obtaining a maize plant from said maize cell.

Generating maize plants with enhanced resistance to northern leaf blight

CRISPR-CAS systems for genome editing

Growing community of inventors

Ankeny, IA, United States of America

Zhenglin Hou