Academy of Military Medical Science, Pla

Zhenghao Zhao

Wei Chen

Zhe Zhang

Qiang Guo

Jinlong Zhang

Lihua Hou

Ling Fu

Shipo Wu

Xiaohong Song

Busen Wang

A single plasmid vector system for packaging recombinant human adenovirus type 4. The vector system contains an E3 region-deleted human adenovirus type 4 (HAdV-4 or Ad4) genome, a vector sequence for amplifying plasmids in bacteria, a pBR322 replication origin, a kanamycin resistance gene, and a replication control sequence; and an exogenous gene embedding site is located behind a packaging signal of the human adenovirus type 4 and in front of an E1 region. The present invention further provides a method for packaging the recombinant human adenovirus type 4 by the single plasmid vector system and an application in vaccine and drug preparation. The vector system can be used for rapidly and efficiently preparing a human adenovirus type 4 vector recombinant virus for stably expressing an exogenous gene, and has a good application prospect in the fields of preparation of a diagnostic kit, a vaccine, a gene therapy kit and/or a tumor therapy drug, etc.

Single plasmid vector system for packaging recombinant human adenovirus type 4 and application thereof

Growing community of inventors

Beijing, China

Zhenghao Zhao