New Brunswick Scientific Co., Inc.

Zheng Zhenbin

David Freedman

Shaul Reuveny

A sparger for use in microcarrier fermentation systems is provided which includes a narrow, vertically extending outer chamber defined by a solid wall and a wall formed by a screen, wherein air or other gas is forced under pressure through a tube to the bottom of the chamber and from there to a distribution member for the release of the gas upwardly into the chamber. The top of the chamber is provided with exit openings which communicate to a point above the surface of the medium. In an alternate embodiment, gas is released at the bottom of an inner chamber defined by a wall formed with a plurality of screened windows.

Sparger for fermentation and tissue culturing vessels

An improved agitator for use with a fermentation and tissue culturing vessel acting as a microcarrier culture vessel is provided. The agitator includes a hollow body assembly having an open end and a closed end and at least one tubulation communicating with the hollow body assembly at a location spaced from the open end thereof, the tubulation having an exit opening oriented such that movement of the agitator and the tubulation causes a suction or draft at the exit opening to cause flow of fluid from the open end of the hollow body assembly through the hollow body assembly and out through the tubulation exit opening. In this way, a microcarrier cell culture may be stirred in a smooth and non-erratic way thereby minimizing the detachment of the rounded growth cells from the microcarriers.

Growing community of inventors

New Brunswick, NJ, United States of America

Zheng Zhenbin