Purdue Research Foundation

Zheng Ouyang

Robert Graham Cooks

Ziqing Lin

Alan Keith Jarmusch

Ahmed Mohamed Hamid

Jiangjiang Liu

He Wang

Nicholas Alan Charipar

Jason David Harper

Nicholas E Manicke

Qian Yang

Chien-hsun Chen

Livia Schiavinato Eberlin

Sandilya Venkata Garimella

Yue Ren

Liang Gao

Linfan Li

Zane Baird

Xin Li

Guangtao Li

Xiaoyu Zhou

Adam Hollerbach

Michael Pugia

Xiao Wang

Wenpeng Zhang

Zhiping Zhang

William Johnson Chappell

Wen-Ping Peng

James Mitchell Wells

Yu Xia

Keyong Hou

Xiaoxiao Ma

Tsung-Chi Chen

Livia Schiavinato

Chen Tsung-Chi

Wei Xu

Michael P Goodwin

Thomas Blake

Ethan R Badman

Morgan McLuckey

Ryan Espy

Wengpeng Zhang

Jianjjiang Liu

Nicholas Charipar

Jason Harper

The invention generally relates to mass tag analysis for rare cells and cell free molecules. In certain embodiments, the invention provides an apparatus including an essentially non-absorbent membrane having at least one pore, a microwell operably associated with the essentially non-absorbent membrane, and an electric field generator. The apparatus may be configured such that an electric field produced by the electric field generator operably interacts with a sample in the microwell and expels a droplet of the sample through the at least one pore in the essentially non-absorbent membrane. In certain embodiments, apparatuses of the invention are used for detection, and optionally quantification, of a target analyte from a heterogeneous sample, such as a rare target analyte (e.g., rare cell) from a biological sample.

Mass tag analysis for rare cells and cell free molecules

The invention generally relates to sample analysis with a miniature mass spectrometer. In certain embodiments, the invention provides methods that involve generating ions of a first analyte and ions of a second analyte. Those ions are transferred through a discontinuous sample introduction interface into a first ion trap of a mass spectrometer in a manner in which the discontinuous sample introduction interface remains open during the transferring. The discontinuous sample introduction interface is closed and the ions are sequentially transferred to a second ion trap of the mass spectrometer where they are sequentially analyzed.

Sample quantitation using a miniature mass spectrometer

The invention generally relates to systems and methods for quantifying an analyte extracted from a sample. In certain embodiments, the invention provides methods that involve introducing a solvent into a capillary, introducing the capillary into a vessel including a sample such that a portion of the sample is introduced into the capillary, moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent, thereby causing the analyte to be extracted from the sample and into the solvent, analyzing the analyte that has been extracted from the sample, and quantifying the analyte. In certain embodiments, the quantifying step is performed without knowledge of a volume of the sample and/or solvent.

Systems and methods for quantifying an analyte extracted from a sample

The invention generally relates to ion generation using modified wetted porous materials. In certain aspects, the invention generally relates to systems and methods for ion generation using a wetted porous substrate that substantially prevents diffusion of sample into the substrate. In other aspects, the invention generally relate to ion generation using a wetted porous material and a drying agent. In other aspects, the invention generally relates to ion generation using a modified wetted porous substrate in which at least a portion of the porous substrate includes a material that modifies an interaction between a sample and the substrate.

Ion generation using modified wetted porous materials

The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.

Enclosed desorption electrospray ionization probes and method of use thereof

The invention generally relates to a sample dispenser including an internal standard and methods of use thereof.

Sample dispenser including an internal standard and methods of use thereof

The invention generally relates to systems and methods for mass spectrometry analysis of microorganisms in samples.

Mass spectrometry analysis of microorganisms in samples

Systems and methods for analyzing an analyte extracted from a sample using an adsorbent material.

Systems and methods for analyzing an analyte extracted from a sample using an adsorbent material

The invention generally relates to methods for analyzing an analyte in a sample that involve providing a capture module, the module configured to capture an analyte from a sample in an ambient environment and generate ions of the analyte, wherein the capture module comprises a cartridge and a porous substrate within the cartridge that is connected to a voltage source; and generating ions of the analyte from the capture module that are analyzed in a mass analyzer operably coupled to the capture module, thereby analyzing the analyte in the sample.

Growing community of inventors

West Lafayette, IN, United States of America

Zheng Ouyang