Massachusetts Institute of Technology

Tianjin Greenbio Material Co., Ltd.

Zheng-Jun Li

Gregory N Stephanopoulos

Qiong Wu

Brian Pereira

Guo-Qiang Chen

Zhen-Yu Shi

Ying-Ying Guo

Jia Jian

Some aspects provide engineered microbes for glycolate production. Methods for microbe engineering and culturing are also provided herein. Such engineered microbes exhibit greatly enhanced capabilities for glycolate production.

Microbial production of renewable glycolate

Known attempts using engineered bacteria to produce P(3HB-co-4HB) with carbon sources that are structurally unrelated to 4-hydroxybutyrate resulted in relatively low 4HB monomer content of 1.5 to 5 mol %. The current invention provides recombinant hosts for producing P(3HB-co-4HB) wherein the plasmid including succinate semialdehyde dehydrogenase gene (sucD gene) and 4-hydroxybutyrate dehydrogenase gene (4hbD gene) further includes pyruvate decarboxylase promoter (P). It was found that the 4HB monomer content in P(3HB-co-4HB) is significantly enhanced to be over 20 mol %, in the range of 8.8 to 23 mol %.

Recombinant hosts and methods for manufacturing polyhydroxyalkanoate

Growing community of inventors

Beijing, China

Zheng-Jun Li