Zhejiang University

Zhejiang Nhu Company Ltd.

Shangyu Nhu Biological Chemical Co., Ltd.

Heilongjiang Nhu Biotechnology Company Ltd.

Zhaofeng Chen

Baishan Hu

Yong Li

Shenfeng Yuan

Yi Min

Qichuan Li

Guisheng Qiu

Hongwei Yu

Xuguang Li

Yongqiang Zhu

Kai Yu

Jinyang Zhang

Yang Yao

Shichun Tu

Yunxuan Luo

The present application relates to a method for extracting coenzyme Q10 and a phospholipid from a coenzyme Q10 fermentation bacterium powder. The method is characterized in that the fermentation bacterium powder of a coenzyme Q10 production strain is subjected to extraction with a mixed solvent of which the three-dimensional Hansen solubility parameter is between 21 and 23 (J/cm)and the hydrogen bonding solubility parameter thereof is between 10 and 12 (J/cm). The present invention can efficiently extract two products, namely coenzyme Q10 and a phospholipid, from the coenzyme Q10 fermentation bacterium powder; the process thereof is highly operable, easy to be industrialized, and can provide a product with high purity and yield, having great economic benefit.

Method for extracting coenzyme Q10 and phospholipid from coenzyme Q10 fermentation bacterial powder

The present application relates to a method for fermentative production of oxidized coenzyme Q10 and high-content oxidized coenzyme Q10 prepared therefrom. For the method for fermentative production of oxidized coenzyme Q10, in a fermentation process of a production strain, the oxidation-reduction potential (ORP) of a fermentation broth is controlled to be &#x2212;50 to 300 Mv, and preferably the oxidation-reduction potential (ORP) of the fermentation broth is controlled to be 50 to 200 mV. By controlling the ORP of the fermentation broth, the method for fermentative production of oxidized coenzyme Q10 enables the oxidized coenzyme Q10 content in the coenzyme Q10 produced by microorganisms to reach 96% or more, and the product is substantially composed of a single component, which makes post-treatment more convenient. Oxidized coenzyme Q10 is more stable than reduced coenzyme Q10, and as compared with the coenzyme Q10 obtained by fermentative production in the prior art, high-content oxidized coenzyme Q10 degrades in a less amount in organisms. In addition, the fermentation method of the present application has a high potency.

Method for fermentative production of oxidized coenzyme Q10

Growing community of inventors

Zhejiang, China

Zhaofeng Chen