United States of America, As Represented by the Secretary of Agriculture

Washington State University

Cold Spring Harbor Laboratory

Uwm Research Foundation, Inc.

Zhanguo Xin

John A Browse

John J Burke

Chaofu Lu

Chad M Hayes

Doreen Ware

Gloria B Burow

Yinping Jiao

Dazhong Zhao

Disclosed herein are nuclear male sterile sorghum mutants, three-component genetic constructs, and methods of using said mutants and genetic constructs, such as in a two-line male sterility system for hybrid breeding.

Sterile mutant and two line breeding system

Aspects of the invention provide methods for differential regulation of fatty acid unsaturation in seed oil and membrane lipids of plants based on modulation of a previously unknown biosynthetic pathway involving a novel phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT) that regulates phosphatidylcholine biosynthesis in developing oil seed plants. Specific aspects relate to inventive PDCT polypeptides including, for example, variants, deletions, muteins, fusion proteins, and orthologs thereof (collectively PDCT proteins), to nucleic acids encoding same, to plants comprising such PDCT sequences or proteins or devoid or depleted of such PDCT proteins or sequences, and to methods for generating plants having altered or no PDCT expression and/or activity, including but not limited to methods comprising mutagenesis, recombinant DNA, transgenics, etc.

Compositions and methods for differential regulation of fatty acid unsaturation in membrane lipids and seed oil

The present disclosure provides nucleic acids that confer a multi-seeded phenotype when expressed in plants and uses thereof.

Sorghum yield enhancement gene

Genetically altered sorghum plants expressing the multi-seeded #2 phenotype contain one of two genomic alterations in the Sb06g018040 gene which result in reduced activity of the encoded protein, a class II 13-lipoxygenase. This phenotype and genotype are referred to as msd2. These alterations result in increased number of seeds and seed weight, thus increasing the yield of the genetically altered plant. These alterations can be generated in the ortholog genes in maize (TS1), rice, barley, and other monocot plants, generating the MSD2 phenotype. The seeds of one particular MSD2has been deposited with ATCC and assigned Accession Number PTA-121634.

Mutant sorghum bicolor having enhanced seed yield

Aspects of the invention provide methods for differential regulation of fatty acid unsaturation in seed oil and membrane lipids of plants based on modulation of a previously unknown biosynthetic pathway involving a novel phosphatidylcholine: diacylglycerol cholinephosphotransferase (PDCT) that regulates phosphatidylcholine biosynthesis in developing oil seed plants. Specific aspects relate to inventive PDCT polypeptides including, for example, variants, deletions, muteins, fusion proteins, and orthologs thereof (collectively PDCT proteins), to nucleic acids encoding same, to plants comprising such PDCT sequences or proteins or devoid or depleted of such PDCT proteins or sequences, and to methods for generating plants having altered or no PDCT expression and/or activity, including but not limited to methods comprising mutagenesis, recombinant DNA, transgenics, etc.

Growing community of inventors

Lubbock, TX, United States of America

Zhanguo Xin