Koninklijke Philips Corporation N.v.

Zeynep Seflek Unay

Stein Kuiper

Sjoerd Stallinga

Roel Penterman

Bas Hulsken

Marc Wilhelmus Gijsbert Ponjee

Sieglinde Neerken

Paul Arnold Van De Wiel

Kristiane Anne Schmidt

Oana Mihaela Piciu

Ron Gill

Bart Edward Gusta Jozef Van Meerbergen

Bo Joakim Isaksson

Mark W G Ponjee

Bart E G J Van Meerbergen

Methods and devices for detection of micro-organisms present or suspected to be present within a mammalian blood sample are provided. A selective lysis is obtained by incubating the sample in a non-ionic detergent under alkaline conditions.

Selective lysis of cells

A method of analyzing a sample fluid containing organic microobjects is proposed. The method comprises the steps of: up-concentrating (S) the microobjects by removing, in a total time T, a volume Vof the sample fluid from the upconcentrate sample microobjects; immersing (S) the microobjects in a transfer fluid, or leaving the microobjects in a remaining portion of the sample fluid, the remaining portion of the sample fluid then providing the transfer fluid; filtering (S), in a total time T, a volume Vof the transfer fluid by a filter, thereby accumulating the microobjects on the filter; and generating (S) an image of the microobjects accumulated on the filter; wherein the throughput V/Tof the step of up-concentrating (S) is greater than the throughput V/T, of the step of filtering (S). The filter may be a second filter, and the step of up-concentrating (S) may involve: filtering the sample fluid by a first filter, thereby accumulating the microobjects on the first filter. An apparatus or system for analyzing a sample fluid containing organic microobjects is also disclosed.

Up-concentration of organic microobjects for microscopic imaging

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Istanbul, Turkey

Zeynep Seflek Unay