Dow Agrosciences LLC

Ze Sheng Li

Donald J Merlo

Timothy Denver Hey

Thomas Meade

Baolong Zhu

Scott Bevan

Scott B Bintrim

Patricia C Apel-Birkhold

Weiting W Ni

Amanda D Schleper

Jon Christopher Mitchell

Jon C Michell

The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable from, and, can be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a 'stand-alone' TC protein (from, or, for example) is enhanced by one or more TC protein 'potentiators' derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-aloneprotein toxin as well as a stand-aloneprotein toxin. (As one skilled in the art knows,toxin proteins tend to be more desirable for controlling lepidopterans whiletoxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.

[object Object]

The subject invention relates to the surprising discovery that toxin complex (TC) proteins, obtainable fromandcan be used interchangeably with each other. In particularly preferred embodiments of the subject invention, the toxicity of a 'stand-alone' TC protein (fromorfor example) is enhanced by one or more TC protein 'potentiators' derived from a source organism of a different genus from which the toxin was derived. As one skilled in the art will recognize with the benefit of this disclosure, this has broad implications and expands the range of utility that individual types of TC proteins will now be recognized to have. Among the most important advantages is that one skilled in the art will now be able to use a single set of potentiators to enhance the activity of a stand-aloneprotein toxin as well as a stand-aloneprotein toxin. (As one skilled in the art knows,toxin proteins tend to be more desirable for controlling lepidopterans whiletoxin proteins tend to be more desirable for controlling coleopterans.) This reduces the number of genes, and transformation events, needed to be expressed by a transgenic plant to achieve effective control of a wider spectrum of target pests. Certain preferred combinations of heterologous TC proteins are also disclosed herein. Other objects, advantages, and features of the subject invention will be apparent to one skilled in the art having the benefit of the subject disclosure.

Mixing and matching TC proteins for pest control

Growing community of inventors

Westfield, IN, United States of America

Ze Sheng Li