Rudjer Boskovic Institute

Industrie Chimiche Capparo S.p.a.

Zdenko Hamersak

Vitomir Sunjic

Ivana Dodig

Mario Cindric

Amir Avdagic

Maja Majeric

Present invention refers to a novel and improved method of derivatization and detection of amino acid sequence and/or identification of proteins, peptides by a new derivatization compound. Precisely, the method discloses a novel approach to derivatization of peptides or proteins by compounds comprising two or more sulfonyl groups and analysis of derivatized analytes in negative mode of operation of mass spectrometer. This method allows unambiguous analysis of amino acid sequence of long-chain peptides/proteins. Also, the invention discloses a novel synthesis procedure of 5-formyl-benzene-1,3-disulphonic acid as derivatization compound.

Method of detection of amino acid sequence and/or identification of peptides and proteins, by use of a new derivatization reagent and synthesis of 5-formyl-benzene-1,3-disulphonic acid as derivatization reagent

A method for manufacturing S-3-(4'-tert-butyl)-phenyl-2-methyl propylamines having the structure I ##STR1## where NR.sub.1 R.sub.2 is a dialkylamine group, pyrrolidine, piperidine, or cis-3,5-dimethyl morpholine, comprising a reduction of the compound having the structure II ##STR2##

Method for the enantioselective synthesis of chiral derivatives of S-3-(4'-

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Zagreb, Croatia

Zdenko Hamersak