Unilever Patent Holdings B.v.

Internationale Octrooi Maatschappij 'octropa' Bv

Smithkline Biologicals, S.a.

Rhein Biotech Gesellschaft Fur Neue Biotechnologische Prozesse Und

Zbigniew A Janowicz

Cornelis P Hollenberg

Cornelis T Verrips

Adrianus M Ledeboer

Jan Maat

Christiaan Visser

Claus Bollschweiler

Alexander Wm Strasser

Pierre Voet

Jurgen Schweden

Michael Piontek

Nigel Harford

Ulrike Weydemann

Armin J Merckelbach

Eric Jacobs

Teresa Cabezon

Martin Comberbach

Apolonia Rutgers

A composite particle comprising at least two polypeptides corresponding to all or part of a protein having the biological activity of one of the hepatitis B surface antigens, wherein the particle presents at least two antigenic determinants provided by the S-protein, preS2-protein or preS1-protein, said particle optionally further comprising host specific lipids. The invention also provides a modified hepatitis B surface antigen L protein which may be used alone or incorporated into such composite particles. A particular embodiment of the modified L protein (L*) comprises residues 12-52 followed by residues 133-145 followed by residues 175-400 of the L protein and a particular embodiment of a composite particle has the form (L*, S) where S is the S-protein of HBsAg. Improved hepatitis B vaccines may be prepared following expression of the above immunogens, especially in yeasts such as S. cerevisiae and Hansenula polymorpha.

Antigens and methods therefor

The structural genes and their regulatory DNA sequences of an alcohol oxidase (MOX) and a dihydroxyacetone synthase (DHAS) of Hansenula polymorpha have been isolated and the nucleotide sequences determined. The invention relates to the use of the MOX gene, as well as the use of the regulatory DNA sequences of MOX and/or DAS in combination with the MOX gene, optionally after modification thereof, or other oxidase genes, or other genes, to produce engineered microorganisms, 0in particular yeasts. Said engineered microorganisms can produce oxidases or other enzymes in yields that allow industrial application on a large scale. Moreover, said engineered microorganisms can produce oxidases having improved properties with respect to their application in oxidation reactions and/or in bleaching and detergent products.

Expression and production of polypeptides using the promoters of the

Recombinant production of proteins in yeast

The structural genes and their regulatory DNA sequences of an alcohol oxidase (MOX) and a dihydroxyacetone synthase (DHAS) of Hansenula polymorpha have been isolated and the nucleotide sequences determined. The invention relates to the use of the MOX gene, as well as the use of the regulatory DNA sequences of MOX and/or DAS in combination with the MOX gene, optionally after modification thereof, or other oxidase genes, or other genes, to produce engineered microorganisms, in particular yeasts. Said engineered microorganisms can produce oxidases or other enzymes in yields that allow industrial application on a large scale. Moreover, said engineered microorganisms can produce oxidases having improved properties with respect to their application in oxidation reactions and/or in bleaching and detergent products.

Regulatory sequences of alcohol oxidase (MOX) and

Growing community of inventors

Erkrath, Germany

Zbigniew A Janowicz