Monsanto Technology LLC

The Samuel Roberts Noble Foundation

Zarir E Vaghchhipawala

Xudong Ye

Elysia K Krieger

Richard J Lawrence

Kirankumar S Mysore

Jianping Xu

The present disclosure is in the field of plant transformation. The disclosure provides methods for increasing the rate of site-directed integration of a sequence of interest in plants. The disclosure also provides methods for increasing the efficiency of Rhizobiales-mediated plant transformation.

Methods and compositions for use in genome modification in plants

The invention provides methods and compositions for increasing the efficiency of genetic transformation of host cells, including plant cells, and other eukaryotic cells, by reducing the expression of a polypeptide active in a pathway, such as the NHEJ pathway, for repairing damage to the cellular genome. In certain embodiments, the polypeptide is active in repairing double strand breaks (DSB's) of a cellular genome, and may include XRCC4, KU70, KU80, the DNA-activated Protein Kinase (DNA-Pkcs), and ATM. Methods for enhancing the resistance of plant cells to Crown Gall disease are also provided. In another aspect, genetic regulatory elements are provided, including an XRCC4 promoter.

Methods and compositions for increasing plant transformation efficiency

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Madison, WI, United States of America

Zarir E Vaghchhipawala