Enzo Life Sciences, Inc.

Enzo Biochem, Inc.

The City University of New York

Enzo Life Sciences, Inc. C/o Enzo Biochem, Inc.

Zaiguo Li

Praveen Pande

Natarajan Raju

Wayne Forrest Patton

Maciej Bernard Szczepanik

Robert Bittman

Yuejun Xiang

Wayne F Patton

Maciej Szczepanik

Elazar S Rabbani

James J Donegan

Jack Coleman

Dee Shen

Elazar Rabbani

Provided are methods for labeling target molecules, such as nucleic acids, with fluorescent dye compounds having the formula One method embodiment includes contacting reactive group Z of the fluorescent dye compound with the target molecule such that reactive group Z reacts with the target molecule to form a covalent bond between the group and the target molecule. Another method embodiment includes contacting a fluorescent dye compound that further includes a first member of a binding pair, with a target molecule that includes a second member of the binding pair. Also provided are target molecules labeled with the fluorescent dye compounds.

Fluorescent dyes and methods of use thereof

The present disclosure provides reactive quencher dyes that can be used in the detection and/or quantification of desirable target molecules, such as proteins, nucleic acids and various cellular organelles. These dyes are essentially non-fluorescent but are efficient quenchers of various fluorescent dyes. Also, provided are methods of using the dyes, bio-probes incorporating dyes and methods of using the bio-probes. The quencher dyes described herein are modified to provide beneficial properties.

Monoazo dyes with cyclic amine as fluorescence quenchers

The invention provides methods for synthesizing the compound (2R,3S,4E)-N-methyl-5-(4'-pentylphenyl)-2-aminopent-4-ene-1,3-diol, also known as SK1-I, and intermediate compounds used in its synthesis.

Methods and intermediates for synthesizing SK1-I

Monoazo dyes with cyclic amine as fluorescence quencers

Provided are various compounds comprising the formula Also provided are fluorescent dyes comprising the above compound. Additionally, a fluorescence energy transfer system is provided that comprises the above-described fluorescent dye and a second dye, wherein the second dye is capable of energy transfer with the fluorescent dye. Further provided is a kit for labeling a target molecule, where the kit comprises the above-described fluorescent dye with additional reagents useful for labeling the target molecule. Additionally provided is a target molecule labeled with the above-described fluorescent dye. Methods of labeling a target molecule with the above-described fluorescent dye are also provided.

Fluorescent dyes

Provided are compounds, methods and kits for identifying in cells of interest organelles including nuclei and a wide variety of organelles other than nuclei (non-nuclear organelles), as well as cell regions or cell domains. These compounds and methods can be used with other conventional detection reagents for identifying the location or position or quantity of organelles and even for distinguishing between organelles in cells of interest.

Fluorochromes for organelle tracing and multi-color imaging

Provided are assays useful for detecting and monitoring autophagy and phospholipidosis, including the progression of lysosomal storage diseases. Drugs and treatments for lysosomal storage diseases can be monitored for effectiveness in lysosomal storage disease conditions. Drug candidates and suspected toxic agents can also be screened for toxicity to cells, tissues and organs. Also provided are methods for distinguishing between phospholipidosis activators and autophagy pathway perturbation agents.

Autophagy and phospholipidosis pathway assays

The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified to provide beneficial properties.

The present invention provides a fluorescent dye that incorporates a pentavalent phosphorus or arsenic into its ring structure. Also provided is a fluorescence energy transfer system that comprises the above fluorescent dye and a second dye, where the second dye is capable of energy transfer with the fluorescent dye. Additionally provided is a kit for labeling a target molecule. In further embodiments, another kit for labeling a target molecule is provided. A target molecule labeled with the above-described fluorescent dye is also provided. In additional embodiments, methods of labeling a target molecule are provided.

Growing community of inventors

Little Neck, NY, United States of America

Zaiguo Li