Irori

Discovery Partners International, Inc.

Nexus Biosystems, Inc.

Irori Technologies, Inc.

Zahra Parandoosh

Gary S David

Xiao-Yi Xiao

Michael P Nova

Andrew E Senyei

Hanan Potash

Chanfeng Zhao

Yozo Satoda

John E Lillig

Anthony W Czarnik

Gordon W Hudson

Robert Neeper

In a multiplexed assay, each molecule of a plurality of molecules is attached to a support matrix with a substrate adapted for attachment and/or synthesis of molecules and an integrally-formed memory device with an optically-encoded identifier to uniquely identify the molecule attached to the substrate. The molecules are exposed to one or more processing conditions then placed within the path of an optical detector adapted to read the optically-encoded identifier and measure biochemical processes on each support matrix. The support matrices may besingulated to be read by the optical detector one at a time.

Multiplexed assay using encoded solid support matrices

Combinations, called matrices with memories, of matrix materials with remotely addressable or remotely programmable recording devices that contain at least one data storage unit are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The matrix materials may additionally include fluophors or other luminescent moieties to produce luminescing matrices with memories. The data storage units are non-volatile antifuse memories or volatile memories, such as EEPROMS, DRAMS or flash memory. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, cell sorting, drug delivery, chemical modification and other uses. Methods for electronically tagging molecules, biological particles and matrix support materials, immunoassays, receptor binding assays, scintillation proximity assays, non-radioactive proximity assays, and other methods are also provided.

Multiplexed assays using encoded solid supports

Combinations, called matrices with memories, of matrix materials that are encoded with an optically readable code are provided. The matrix materials are those that are used in as supports in solid phase chemical and biochemical syntheses, immunoassays and hybridization reactions. The matrix materials may additionally include fluophors or other luminescent moieties to produce luminescing matrices with memories. The memories include electronic and optical storage media and also include optical memories, such as bar codes and other machine-readable codes. By virtue of this combination, molecules and biological particles, such as phage and viral particles and cells, that are in proximity or in physical contact with the matrix combination can be labeled by programming the memory with identifying information and can be identified by retrieving the stored information. Combinations of matrix materials, memories, and linked molecules and biological materials are also provided. The combinations have a multiplicity of applications, including combinatorial chemistry, isolation and purification of target macromolecules, capture and detection of macromolecules for analytical purposes, selective removal of contaminants, enzymatic catalysis, cell sorting, drug delivery, chemical modification and other uses. Methods for tagging molecules, biological particles and matrix support materials, immunoassays, receptor binding assays, scintillation proximity assays, non-radioactive proximity assays, and other methods are also provided.

Method for tagging and screening molecules

Methods for irradiation induced graft polymerization of a monomers, such as styrenes, onto fluoropolymers are provided. The methods, which involve either the use of acids, preferably mineral acids, or creating a rough surface on the fluoropolymer, provide higher levels of grafting of the copolymer than grafting in the absence of the acid or of the rough surface on the fluoropolymer. Also provided are grafted copolymers produced by the methods. Methods for increasing the performance of solid phase assays, such as scintillation proximity assays, are also provided.

Radiation-grafted solid supports for chemical synthesis

Remotely programmable matrices with memories and uses thereof

Matrices with memories and uses thereof

A method is for determining the number of cells in cell culture is provided. In practicing the method, the cells are lysed and incubated at about 18.degree. C. to 25.degree. C. at acid pH with a benzothiazole substrate for a sufficient time to generate detectable fluorescence that is proportional to cell number, and then measuring fluorescence. The method is highly sensitive and can detect small differences or changes in cell number. Thus, the method can be used in drug screening assays. An automated high throughput screening assay for identifying compounds that modulate angiogenesis using the method is also provided.

Fluorescence-based assays for measuring cell proliferation

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San Diego, CA, United States of America

Zahra Parandoosh