The United States of America As Represented by the Administrator of the U.S. Environmental Protection Agency

Yvette R Roybal-McKenna

Kristen P Brenner

Clifford C Rankin

Alfred P Dufour

An improved method for detection of total coliforms andcomprising a broth containing an ingredient that will suppress growth of non-coliform gram negative bacteria, and at least one chromogen or fluorogen has been used effectively and is cost effective. Preferred methods include use of filter and/or plates containing the growth-promoting ingredients and the indicators.

[object Object]

An improved method for detection of total coliforms and comprising a broth containing an ingredient that will encourage growth and repair of injured coliforms, buffers to maintain a pH in the range of 6.5-8, at least one agent that suppresses growth of gram positive cocci and spore-forming organisms, at least one active agent that will suppress growth of non-coliform gram negative bacteria, and at least one chromogen or fluorogen has been used effectively and is cost effective. In the preferred embodiment, both a fluorogen and chromogen were used. Preferred methods include use of filter and/or plates containing the growth-promoting ingredients and the indicators.

Method for detection of total coliforms and E. coli

An improved method for detection of total coliforms and,comprising a broth containing an ingredient that will encourage growth and repair of injured coliforms, buffers to maintain a pH in the range of 6.5-8, at least one agent that suppresses growth of gram positive cocci and spore-forming organisms, at least one active agent that will suppress growth of non-coliform gram negative bacteria, and at least one chromogen or fluorogen has been used effectively and is cost effective. In the preferred embodiment, both a fluorogen and chromogen were used. Preferred methods include use of filter and/or plates containing the growth-promoting ingredients and the indicators.

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Gilbert, AZ, United States of America

Yvette R Roybal-McKenna