The United States of America As Represented by the Administrator of the

Yvette R Roybal

Kristen P Brenner

Clifford C Rankin

Alfred P Dufour

An improved method for detection of total coliforms and E. coli comprising placing the target sample in a broth containing an ingredient that will encourage growth and repair of injured coliforms, at least one agent that suppresses growth of gram positive cocci and spore-forming organisms, at least one active agent that will suppress growth of non-coliform gram negative bacteria, and at least one chromogen or fluorogen has been used effectively and is cost effective. In the preferred embodiment, both a fluorogen and chromogen were used. Preferred methods include use of filter and/or plates containing the growth-promoting ingredients and the indicators.

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Lakewood, CO, United States of America

Yvette R Roybal